Class 1 alleles (loss-of-function) n526n540ird : precocious heterochronic lineage alterations in ectoderm; abnormal development of vulva endoderm and mesoderm; abnormal cuticle formation; ES2; male more severely affected only three molts gonadal development abnormal. Similar phenotype in n536n540/Df. NA > 10 (n179ts etc.; also Class 1a n355n679ird ts (only early events precocious); Class 1b n360ts (only late events precocious). Class 2 alleles (gain-of-function) n536sd : retarded heterochronic alterations in many lineages abnormal vulval development cuticle formation supernumerary molts extra divisions in sex mesoblasts intestine etc.; gonadal lineages normal. ES3. ME0? NA2 (n355sd). For both Class 1 and Class 2 mutations late lineages are less affected if animal develops via dauer stage.
[C.elegansII] n526n540ird : lf allele (Class II, a-b-), precocious heterochronic lineage alterations in ectoderm; abnormal development of vulva,endoderm and mesoderm; abnormal cuticle formation; precocious dauer entry at L1 molt; ES2; male more severely affected, only three molts, gonadal development abnormal. Similar phenotype in n536n540/Df. OA>10: n179ts, etc.; also n355n679ird,ts (Class V, a-b+, only early events precocious); n360ts(Class III, a+b-, only late events precocious). Also gain-of-function alleles: n536sd (Class I, retarded heterochronic alterations in many lineages; abnormal vulval development, cuticle formation, supernumerary molts, extra divisions in sex mesoblasts, intestine etc.; gonadal lineages normal. ES3), n355sd(similar). Numerous intragenic revertants. For both lf and gf mutations, late lineages are less affected if animal develops via dauer stage. Cloned: two 3.5 kb transcripts, differing 5',encoding 70 kD novel proteins; antibodies stain nuclei; protein levels high in L1, reduced or absent later. Gain-of-function mutations affect3' UTR. [Ruvkun and Giusto 1989; Wightman et al. 1993; GR; VT]
lin-14 encodes a novel protein whose activity is required for specifying the division timings of a specific group of cells during postembryonic development; lin-14 loss-of-function mutations result in the premature appearance of later larval lineages, while gain-of-function mutations result in reiteration of L1 larval stages lineages; in addition, lin-14 acts as a positive regulator of AVM, PVM, and FLP touch cell development; in regulating developmental timing, lin-14 acts, in part, by positively regulating the activity of lin-28, which encodes a cytoplasmic protein also required for proper developmental timing; at hatching, LIN-14 is detected in the nuclei of blast cells and neurons; later, from the late L1 to adult stages, LIN-14 levels are negatively regulated by translational repression mediated by lin-4, a 22-nt small temporal RNA (stRNA) that is complementary to sequences in the lin-14 3' UTR.
Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity and single-stranded DNA binding activity. Involved in several processes, including negative regulation of transcription by RNA polymerase II; regulation of cell fate specification; and regulation of nematode larval development, heterochronic. Located in condensed nuclear chromosome. Expressed in several structures, including QL; QR; neurons; oocyte; and somatic nervous system.