WormBase Tree Display for Antibody: WBAntibody00000337
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| WBAntibody00000337 | Summary | Polyclonal rabbit antibodies against recombinant protein. These isoform-specific antibodies recognize exon 4 (unique to LIN-14A). | ||
|---|---|---|---|---|
| Public_name | [cgc4497]::anti-LIN-14_a | |||
| Gene | WBGene00003003 | |||
| Isolation | Original_publication | WBPaper00004497 | ||
| Location | GR | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | PCR fragments containing exon 4 of lin-14 were generated and subcloned in frame downstream of glutathione S-transferase (GST) in pGEX-2T using BamHI and EcoRI sites in the primers. Fusion proteins were induced in the host strain DH5. Soluble GST-exon 3 fusion protein was affinity purified on glutathione Sepharose 4B (Pharmacia, Piscataway, NJ) and used to immunize rabbits (Charles River Pharmservices). Anti-LIN-14A antibodies were purified with His6:LIN-14A. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2427 | |||
| Reference | WBPaper00004497 |
