WormBase Tree Display for Construct: WBCnstr00019564
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WBCnstr00019564 | Public_name | pVT333G |
---|---|---|
Other_name | p14GFP | |
Summary | [lin-14::gfp] | |
Gene | WBGene00003003 | |
Fusion_reporter | GFP | |
Construction_summary | A 5.7-kb EcoRI fragment from cosmid KE7 containing lin-14 exons 4 to 13 plus about 1.7 kb of the lin-14 3'untranslated region (3'UTR) was first cloned into the EcoRI site of pBluescript to make pB14R. An internal EcoRI site within this fragment was eliminated by partial digestion and end-filling during the cloning. The green fluorescent protein (GFP) coding sequence was cut from pPD95.02 with AgeI and SmaI and then blunted and cloned into a blunted EcoNI site of pB14R (with GFP sequences in frame with the carboxy end of lin-14 coding sequence) to make pB14RGFP. p14GFP was made by inserting a 12.8-kb KpnI-SalI fragment from cosmid KE7 into the corresponding sites of pB14RGFP. | |
Reference | WBPaper00003931 |