WormBase Tree Display for Expr_pattern: Expr2229
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| Expr2229 | Expression_of | Gene | WBGene00004920 | |
|---|---|---|---|---|
| WBGene00004914 | ||||
| WBGene00004915 | ||||
| WBGene00004916 | ||||
| WBGene00004917 | ||||
| WBGene00004918 | ||||
| WBGene00004919 | ||||
| Reflects_endogenous_expression_of | WBGene00004914 | |||
| WBGene00004915 | ||||
| WBGene00004916 | ||||
| WBGene00004917 | ||||
| WBGene00004918 | ||||
| WBGene00004919 | ||||
| WBGene00004920 | ||||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005175 | Certain | ||
| GO_term | GO:0043186 | |||
| Subcellular_localization | With most of these antibodies, staining was detected in nuclei and in the cytoplasm of many cell types, consistent with the localization of snRNPs in other organisms. Strikingly, Sm antibodies also stained cytoplasmic particles that colocalized with the P granule component PGL-1 within germ cells and their embryonic precursors. Sm staining was observed in nucleus-associated (perinuclear) P granules of adult germ cells and older embryos, as well as in cytoplasmic P granules of oocytes and early embryos. P granules were stained by several monoclonal and polyclonal antibodies that recognize different epitopes on Sm proteins . Furthermore, the staining of P granules by anti-Sm antibodies was greatly reduced after RNAi of Sm proteins but not after RNAi of RNA polymerase II. In addition, antibodies to U2AF65, another splicing factor, stained nuclei but did not stain P granules. Therefore, these results show that at least some Sm proteins are specific and constitutive components of P granules. | |||
| Type | Antibody | To determine the localization of Sm proteins in embryos and germ cells, embryos and adult gonads was stained with several different Sm antibodies. For staining of Sm proteins, antibodies used included the monoclonal antibodies Y12, 7.13, KSm4, and KSm6 [S18S20]. An autoimmune human anti-Sm serum and polyclonal rabbit antibodies to SmF and SmG were also used [S21, S22]. | ||
| Pattern | Expressed in embryos and adult gonads. Expression pattern in other tissue or life stages are not described in this paper. | |||
| Picture | WBPicture0000009729 | |||
| Reference | WBPaper00005457 | |||
| Antibody_info | WBAntibody00000549 | |||
| WBAntibody00000550 | ||||
| WBAntibody00000551 | ||||
| WBAntibody00000552 | ||||
| WBAntibody00000553 | ||||
| WBAntibody00000554 | ||||
| WBAntibody00000555 |
