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WormBase Tree Display for Variation: WBVar00274962

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Name Class

WBVar00274962EvidencePaper_evidenceWBPaper00005044
NamePublic_namev33
HGVSgCHROMOSOME_II:g.8124162_8125040del
Sequence_detailsSMapS_parentSequenceC41C4
Flanking_sequencesccgtatcccttccatttattacgttggcttagtttgatgaaaagaatcgtggaaagacctg
Mapping_targetC41C4
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00033316
LaboratoryRE
StatusLive
AffectsGeneWBGene00002147
TranscriptC41C4.4a.1VEP_consequencesplice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
VEP_impactHIGH
cDNA_position?-600
CDS_position?-530
Protein_position?-177
Intron_number2-4/11
Exon_number1-5/12
InteractorWBInteraction000003954
WBInteraction000003955
WBInteraction000051435
WBInteraction000500231
WBInteraction000520460
WBInteraction000520461
WBInteraction000520462
WBInteraction000520656
WBInteraction000536400
WBInteraction000536417
WBInteraction000536420
WBInteraction000536537
WBInteraction000536538
WBInteraction000536539
WBInteraction000536540
WBInteraction000536541
WBInteraction000536542
WBInteraction000536543
WBInteraction000536544
WBInteraction000536545
WBInteraction000536546
WBInteraction000536547
WBInteraction000536548
WBInteraction000536549
WBInteraction000536550
WBInteraction000536551
WBInteraction000536552
WBInteraction000536553
WBInteraction000536554
WBInteraction000536555
WBInteraction000536556
WBInteraction000536557
WBInteraction000536558
WBInteraction000536559
WBInteraction000536560
WBInteraction000536561
WBInteraction000536562
WBInteraction000536563
WBInteraction000536564
WBInteraction000536565
WBInteraction000536566
WBInteraction000536567
WBInteraction000536568
WBInteraction000536569
WBInteraction000536570
WBInteraction000536571
WBInteraction000536572
WBInteraction000536573
WBInteraction000536574
WBInteraction000536575
WBInteraction000536576
WBInteraction000536577
WBInteraction000536578
WBInteraction000536579
WBInteraction000536580
WBInteraction000536581
WBInteraction000536582
WBInteraction000536583
WBInteraction000536584
WBInteraction000536585
WBInteraction000536586
WBInteraction000536587
WBInteraction000536588
WBInteraction000536589
WBInteraction000536590
WBInteraction000536591
WBInteraction000536592
WBInteraction000536593
WBInteraction000536594
WBInteraction000536595
WBInteraction000536596
WBInteraction000536597
WBInteraction000536598
WBInteraction000536599
WBInteraction000536600
WBInteraction000536601
WBInteraction000536602
WBInteraction000536603
WBInteraction000536604
WBInteraction000536605
WBInteraction000536606
WBInteraction000536607
WBInteraction000536608
WBInteraction000536609
WBInteraction000536610
WBInteraction000536611
WBInteraction000536612
WBInteraction000536613
WBInteraction000536614
WBInteraction000536615
WBInteraction000536616
WBInteraction000536617
WBInteraction000536618
WBInteraction000536619
WBInteraction000536620
WBInteraction000536621
WBInteraction000536622
WBInteraction000536623
WBInteraction000536624
WBInteraction000536625
WBInteraction000536626
WBInteraction000536627
WBInteraction000536628
WBInteraction000536629
WBInteraction000536630
WBInteraction000536631
WBInteraction000536632
WBInteraction000536633
WBInteraction000536634
WBInteraction000536635
WBInteraction000536636
WBInteraction000536637
WBInteraction000536638
WBInteraction000536639
WBInteraction000536640
WBInteraction000536641
WBInteraction000536642
WBInteraction000536643
WBInteraction000536644
WBInteraction000536645
WBInteraction000536646
WBInteraction000536647
WBInteraction000536648
WBInteraction000536649
WBInteraction000536650
WBInteraction000536651
WBInteraction000536652
WBInteraction000536653
WBInteraction000536654
WBInteraction000536655
WBInteraction000536656
WBInteraction000536657
WBInteraction000536658
WBInteraction000536659
WBInteraction000536660
WBInteraction000536661
WBInteraction000536757
WBInteraction000536758
WBInteraction000536766
WBInteraction000536819
WBInteraction000537510
IsolationMutagenEMS
GeneticsInterpolated_map_positionII0.694564
DescriptionPhenotype (20)
Phenotype_not_observedWBPhenotype:0000062Paper_evidenceWBPaper00005044
Curator_confirmedWBPerson712
Remarkire-1(v33) mutants were viable.Paper_evidenceWBPaper00005044
Curator_confirmedWBPerson712
WBPhenotype:0000114Paper_evidenceWBPaper00036076
WBPaper00041065
Curator_confirmedWBPerson2987
Remark"To determine whether the transcription of rnf-121 is regulated by PEK-1 and the UPR pathway in C . elegans , we performed a real-time PCR analysis of the mutant strains pek-1 ( ok275 ) , ire-1 ( v33 ) , and atf-6 ( ok551 ) , as well as of wild-type worms , treated with the UPR inducers DTT , tunicamycin , and thapsigargin . Although the mRNA levels of hsp-4 were induced upon tunicamycin or DTT treatment and in pek-1 ( ok275 ) and atf-6 ( ok551 ) mutant backgrounds , and abolished in ire-1 ( v33 ) as shown previously ( Shen et al. , 2001 ) , the levels of rnf-121 mRNA were largely unaffected ( Figure 3B ) ."Paper_evidenceWBPaper00036076
Curator_confirmedWBPerson2987
The ire-1(v33) mutation did not affect mRNA levels of genes 4R79.2, Y39G10AR.8, rpl-1, ZK1098.4, and cpl-1 (Table 1)Paper_evidenceWBPaper00041065
Curator_confirmedWBPerson2987
WBPhenotype:0000137Paper_evidenceWBPaper00041065
Curator_confirmedWBPerson2987
Remark"TM (tunicamycin) induction of F48E8.6 did not require ire-1, atf-6, or pek-1, suggesting that its induction did not require any single UPR pathway or that it uses an unconventional UPR pathway (Fig. 1B)."Paper_evidenceWBPaper00041065
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00041065
Curator_confirmedWBPerson2987
WBPhenotype:0000424Paper_evidenceWBPaper00042060
Curator_confirmedWBPerson2987
Remark"Mutants of the two major components of the UPR pathway in C. elegans, ire-1 and xbp-1 (49), were fully sensitive to levamisole and had normal levels of L-AChRs at the NMJ based on immunostaining (Fig. S5 A and B)." (Antibody staining of UNC-38 was normal)Paper_evidenceWBPaper00042060
Curator_confirmedWBPerson2987
WBPhenotype:0000845Paper_evidenceWBPaper00042060
Curator_confirmedWBPerson2987
Remark"Mutants of the two major components of the UPR pathway in C. elegans, ire-1 and xbp-1 (49), were fully sensitive to levamisole and had normal levels of L-AChRs at the NMJ based on immunostaining (Fig. S5 A and B)."Paper_evidenceWBPaper00042060
Curator_confirmedWBPerson2987
WBPhenotype:0001006Paper_evidenceWBPaper00033126
Curator_confirmedWBPerson2021
RemarkWhen the worms were fed with bacteria, the pumping rate in ire-1(v33) animals was only slightly decreased (not statistically significant) compared to WT animalsPaper_evidenceWBPaper00033126
Curator_confirmedWBPerson2021
WBPhenotype:0001990Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
WBPhenotype:0002423Paper_evidenceWBPaper00049307
Curator_confirmedWBPerson2987
Remark"Mutation of the ER-UPR genes ire-1 and xbp-1 did not suppress Neuro-Nmnat1(gcIs30[Neuro-m-nonN-Nmnat1]) hypoxic survival." (Figure S3b)Paper_evidenceWBPaper00049307
Curator_confirmedWBPerson2987
EQ_annotationsGO_termGO:0001666PATO:0000460Paper_evidenceWBPaper00049307
Curator_confirmedWBPerson2987
Phenotype_assayGenotypegcIs30 [Neuro-m-nonN-Nmnat1]Paper_evidenceWBPaper00049307
Curator_confirmedWBPerson2987
Reference (13)
RemarkA 878 bp deletion extending from 199 bp upstream of the ATG start codon to bp 679 of ire-1 gene.Paper_evidenceWBPaper00005044
MethodDeletion_allele