The integrated form of DAF-16::GFP (IsDAF-16::GFP) results in a much stronger Daf-c phenotype than non-integrated transgenes. Dauers appear to be partial dauers.
Overexpression of daf-16 with a functional translational fusion of DAF-16 with green fluorescent protein (DAF-16::GFP) slows developmental progression, such that most animals are in the L4 stage after 72 hr (Table 1)
Selenium treatment results in nuclear localization of DAF-16::GFP. The overexpression of DAF-16::GFP also decreases the extent of movement decline in response to selenium treatment.