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WormBase Tree Display for Variation: WBVar00275456

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Name Class

WBVar00275456EvidencePaper_evidenceWBPaper00005036
NamePublic_namezc14
Other_nameCE45635:p.Gly381Arg
CE35836:p.Gly723Arg
C41C4.4a.1:c.2167G>A
C41C4.4b.1:c.1141G>A
HGVSgCHROMOSOME_II:g.8113556C>T
Sequence_detailsSMapS_parentSequenceC41C4
Flanking_sequencestatgtgttaacctcgggtactcatccttttgaaaatcattgcacagacaagcaaatattg
Mapping_targetC41C4
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00005036
Author_evidenceAndrew Murley
Laboratory_evidenceAGD
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00034062
LaboratorySJ
StatusLive
AffectsGeneWBGene00002147
TranscriptC41C4.4b.1 (12)
C41C4.4a.1 (12)
Interactor (11)
GeneticsInterpolated_map_positionII0.679673
DescriptionPhenotypeWBPhenotype:0000081Paper_evidenceWBPaper00049705
Curator_confirmedWBPerson34124
Remarkreduced recovery after prolonged L1 arrestPaper_evidenceWBPaper00049705
Curator_confirmedWBPerson34124
WBPhenotype:0000137Paper_evidenceWBPaper00040397
Curator_confirmedWBPerson2987
RemarkThe ire-1(zc14) mutation suppressed the tunicamycin-induced increase in mRNA expression of uggt-1 and hsp-4Paper_evidenceWBPaper00040397
Curator_confirmedWBPerson2987
WBPhenotype:0001278Paper_evidenceWBPaper00005432
WBPaper00030999
Curator_confirmedWBPerson712
WBPerson2987
Remarkhsp-4::GFP was not induced in untreated or Cd++ treated animals.Paper_evidenceWBPaper00005432
Curator_confirmedWBPerson712
ire-1(zc14) suppressed the expression of hsp-4::GFP induced by cdc-48.1(RNAi);cdc-48.2(RNAi)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
ire-1(zc14) suppressed the expression of hsp-4::GFP induced by hrd-1(RNAi) (Figure 4A)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
ire-1(zc14) suppressed the expression of hsp-4::GFP induced by ufd-1(RNAi) (Figure 4A)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
Phenotype_assayGenotypehsp-4::gfp; cdc-48.1(RNAi); cdc-48.2(RNAi)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
hsp-4::gfp; hrd-1(RNAi)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
hsp-4::gfp; ufd-1(RNAi)Paper_evidenceWBPaper00030999
Curator_confirmedWBPerson2987
WBPhenotype:0001719Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
RemarkInduction of Phsp-4::GFP expression by either hypoxia or tunicamycin was blocked virtually completely by a loss-of-function mutation in ire-1 [ire-1(lf)].Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
WBPhenotype:0001834Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Remark"All three ire-1 alleles failed to produce detectable levels of spliced XBP-1 under normal conditions or after an HP incubation (Fig. 6D and E)."Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
WBPhenotype:0001838Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
RemarkInduction of Phsp-4::GFP expression by either hypoxia or tunicamycin was blocked virtually completely by a loss-of-function mutation in ire-1 [ire-1(lf)].Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
WBPhenotype:0001990Paper_evidenceWBPaper00032521
WBPaper00037064
Curator_confirmedWBPerson712
WBPerson2987
RemarkAnimals were weakly resistant.Paper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
"As previously reported (1), the missense allele ire-1(zc14) was significantly hypoxia resistant (Fig. 5A)."Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
RecessivePaper_evidenceWBPaper00032521
Curator_confirmedWBPerson712
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
WBPhenotype:0002422Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Remark"Indeed, in an assay of Tm-induced developmental arrest, both zc14 and tm400 homozygotes and v33 heterozygotes were Tm resistant, whereas v33 homozygotes were Tm hypersensitive, as had been reported previously (Fig. 5C) (48).Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
WBPhenotype:0002423Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
RemarkTunicamycin (Tm) preconditioning in wild type worms leads to increased tolerance to hypoxia. "... three loss-of-function mutant alleles of ire-1 (Fig. 3B), two alleles of atf-6 (Fig. 3D), and an allele of xbp-1 (Fig. 3F) all were defective for Tm preconditioning (Fig. 2D)."Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0001653Paper_evidenceWBPaper00005432
Curator_confirmedWBPerson712
RemarkMutant animals tolerated cadmium exposure for extended periods of time.Paper_evidenceWBPaper00005432
Curator_confirmedWBPerson712
WBPhenotype:0002423Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Remark"HP (hypoxic preconditioning) consistently provided protection from subsequent harsh hypoxic exposure for wild-type animals (Fig. 4A and B)... unlike the case for Tm preconditioning, ire-1(zc14), a missense mutation in the kinase domain that is thought to abolish the XBP-1 endonuclease activity of IRE-1 and behaves as a reduction-of-function allele (Fig. 3B) (8), did not block HP (Fig. 4C)."Paper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
Variation_effectHypomorph_reduction_of_functionPaper_evidenceWBPaper00037064
Curator_confirmedWBPerson2987
ReferenceWBPaper00040397
WBPaper00032521
WBPaper00037064
WBPaper00005036
WBPaper00005432
WBPaper00030999
WBPaper00049705
WBPaper00050679
WBPaper00065802
RemarkUpdated the mutation to G->A rather than the originally reported G->C based on new sequencing data.Curator_confirmedWBPerson1983
MethodSubstitution_allele