WormBase Tree Display for Variation: WBVar00090682

WBVar00090682 Name: Public_name: n3314
  Sequence_details: SeqStatus: Not_sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00027366
      WBStrain00027389
    Laboratory: MT
    Status: Live
  Affects: Gene: WBGene00003387
  Description: Phenotype: WBPhenotype:0000018 Paper_evidence: WBPaper00050739
        Curator_confirmed: WBPerson712
        Remark: Mutations to the C. elegans serotonin reuptake transporter, mod-5, lead to an accumulation of serotonin at the synaptic cleft, which results in a significant increase in baseline pharyngeal pumping frequency in two out of two experiments. Paper_evidence: WBPaper00050739
            Curator_confirmed: WBPerson712
        Image: WBPicture0000013536 Paper_evidence: WBPaper00050739
            Curator_confirmed: WBPerson712
        Phenotype_assay: Strain: WBStrain00027366 Paper_evidence: WBPaper00050739
              Curator_confirmed: WBPerson712
      WBPhenotype:0000023 Paper_evidence: WBPaper00056790
        Curator_confirmed: WBPerson712
        Remark: animals are hypersensitive to serotonin-induced paralysis Paper_evidence: WBPaper00056790
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00004929 Paper_evidence: WBPaper00056790
              Curator_confirmed: WBPerson712
      WBPhenotype:0000181 Paper_evidence: WBPaper00031671
        Curator_confirmed: WBPerson2021
        Remark: Short dorsal (17%) and subventral (7%) processes were found in the mod-5 mutant Paper_evidence: WBPaper00031671
            Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00031671
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0003666 PATO:0000460 Paper_evidence: WBPaper00031671
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: zdIs13 [ tph-1p::GFP] Paper_evidence: WBPaper00031671
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000381 Paper_evidence: WBPaper00031915
        Curator_confirmed: WBPerson2021
        Remark: Animals were resistant to further fat reduction by fluoxetine treatment. Fluoxetine is a serotonin-specific reuptake inhibitor Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Fat content was visualized by Nile red staining Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000424 Paper_evidence: WBPaper00036766
        Curator_confirmed: WBPerson712
        Remark: 5-HT immunoreactivity was observed in NSMs, ADFs, and adult HSNs but not in AIMs and RIH. Paper_evidence: WBPaper00036766
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006814 PATO:0000460 Paper_evidence: WBPaper00036766
                Curator_confirmed: WBPerson712
            WBbt:0003928 PATO:0000460 Paper_evidence: WBPaper00036766
                Curator_confirmed: WBPerson712
      WBPhenotype:0000631 Paper_evidence: WBPaper00036766
        Curator_confirmed: WBPerson712
        Remark: Mutants remained sensitive to fluoxetine in the paralysis assay, although their sensitivities were slightly reduced compared to WT animals. | Fluoxetine caused muscle relaxation in mod-5 mutants. Paper_evidence: WBPaper00036766
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00005058 Paper_evidence: WBPaper00036766
              Curator_confirmed: WBPerson712
      WBPhenotype:0001183 Paper_evidence: WBPaper00031915
        Curator_confirmed: WBPerson2021
        Remark: Animals deficient in mod-5, a 5-HT reuptake transporter, displayed reduced fat levels relative to wild-type animals Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Fat content was visualized by Nile red staining Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001444 Paper_evidence: WBPaper00032335
        Curator_confirmed: WBPerson712
        Remark: Animals showed defects in gustatory plasticity. Paper_evidence: WBPaper00032335
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were tested for response to 25 mM NaCl after various pre-treatments: a 15-min wash in CTX buffer with or without 100 mM NaCl (liquid), or 30 min on a CTX plate with or without 100 mM NaCl, and in the presence or absence of bacteria, 500 mM glycerol, or 3 uL of benzaldehyde. Paper_evidence: WBPaper00032335
              Curator_confirmed: WBPerson712
      WBPhenotype:0001602 Paper_evidence: WBPaper00031241
        Curator_confirmed: WBPerson712
        Remark: Methiothepin did not extend the life-span of animals (12% increase, n=171) as much as it extended the life-span of N2 animals (28% increase, n=273). Paper_evidence: WBPaper00031241
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were exposed to 10uM methiothepin on day 1 of adulthood. Paper_evidence: WBPaper00031241
              Curator_confirmed: WBPerson712
      WBPhenotype:0001604 Paper_evidence: WBPaper00031241
        Curator_confirmed: WBPerson712
        Remark: Mianserin did not extend the life-span of animals (2% increase, n=217) as it does with N2 animals (31% increase, n=1180). Paper_evidence: WBPaper00031241
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were exposed to 50uM mianserin starting day 1 of adulthood. Paper_evidence: WBPaper00031241
              Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000643 Paper_evidence: WBPaper00036766
        Curator_confirmed: WBPerson712
        Remark: Mutants grown on NGM plates exhibited superficially normal locomotion. Paper_evidence: WBPaper00036766
            Curator_confirmed: WBPerson712
      WBPhenotype:0001462 Paper_evidence: WBPaper00032335
        Curator_confirmed: WBPerson712
        Remark: Animals exhibited wild-type chemotaxis to NaCl. Paper_evidence: WBPaper00032335
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003571 Paper_evidence: WBPaper00032335
              Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were tested for response to 25 mM NaCl. Paper_evidence: WBPaper00032335
              Curator_confirmed: WBPerson712
      WBPhenotype:0001811 Paper_evidence: WBPaper00031915
        Curator_confirmed: WBPerson2021
        Remark: mod-5(n3314) mutants were further susceptible to the fat-reducing effects of exogenously administered serotonin Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Reduced fat content of 5-HT-treated animals was visualized by Nile red staining and confirmed by thin-layer chromatography (TLC) quantitation of total triglycerides extracted from vehicle- and 5-HT-treated worms and by Sudan black fat staining Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
  Disease_info: Models_disease: DOID:809
    Models_disease_in_annotation: WBDOannot00000682
  Reference (18)
  Method: Allele