WormBase Tree Display for Variation: WBVar00090682
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WBVar00090682 | Name | Public_name | n3314 | ||||||
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Sequence_details | SeqStatus | Not_sequenced | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00027366 | ||||||||
WBStrain00027389 | |||||||||
Laboratory | MT | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00003387 | |||||||
Description | Phenotype | WBPhenotype:0000018 | Paper_evidence | WBPaper00050739 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mutations to the C. elegans serotonin reuptake transporter, mod-5, lead to an accumulation of serotonin at the synaptic cleft, which results in a significant increase in baseline pharyngeal pumping frequency in two out of two experiments. | Paper_evidence | WBPaper00050739 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Image | WBPicture0000013536 | Paper_evidence | WBPaper00050739 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Strain | WBStrain00027366 | Paper_evidence | WBPaper00050739 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000023 | Paper_evidence | WBPaper00056790 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | animals are hypersensitive to serotonin-induced paralysis | Paper_evidence | WBPaper00056790 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00004929 | Paper_evidence | WBPaper00056790 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000181 | Paper_evidence | WBPaper00031671 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | Short dorsal (17%) and subventral (7%) processes were found in the mod-5 mutant | Paper_evidence | WBPaper00031671 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Variation_effect | Null | Paper_evidence | WBPaper00031671 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0003666 | PATO:0000460 | Paper_evidence | WBPaper00031671 | ||||
Curator_confirmed | WBPerson2021 | ||||||||
Phenotype_assay | Genotype | zdIs13 [ tph-1p::GFP] | Paper_evidence | WBPaper00031671 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0000381 | Paper_evidence | WBPaper00031915 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | Animals were resistant to further fat reduction by fluoxetine treatment. Fluoxetine is a serotonin-specific reuptake inhibitor | Paper_evidence | WBPaper00031915 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Phenotype_assay | Treatment | Fat content was visualized by Nile red staining | Paper_evidence | WBPaper00031915 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0000424 | Paper_evidence | WBPaper00036766 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | 5-HT immunoreactivity was observed in NSMs, ADFs, and adult HSNs but not in AIMs and RIH. | Paper_evidence | WBPaper00036766 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0006814 | PATO:0000460 | Paper_evidence | WBPaper00036766 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0003928 | PATO:0000460 | Paper_evidence | WBPaper00036766 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000631 | Paper_evidence | WBPaper00036766 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mutants remained sensitive to fluoxetine in the paralysis assay, although their sensitivities were slightly reduced compared to WT animals. | Fluoxetine caused muscle relaxation in mod-5 mutants. | Paper_evidence | WBPaper00036766 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00005058 | Paper_evidence | WBPaper00036766 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001183 | Paper_evidence | WBPaper00031915 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | Animals deficient in mod-5, a 5-HT reuptake transporter, displayed reduced fat levels relative to wild-type animals | Paper_evidence | WBPaper00031915 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Phenotype_assay | Treatment | Fat content was visualized by Nile red staining | Paper_evidence | WBPaper00031915 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
WBPhenotype:0001444 | Paper_evidence | WBPaper00032335 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals showed defects in gustatory plasticity. | Paper_evidence | WBPaper00032335 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were tested for response to 25 mM NaCl after various pre-treatments: a 15-min wash in CTX buffer with or without 100 mM NaCl (liquid), or 30 min on a CTX plate with or without 100 mM NaCl, and in the presence or absence of bacteria, 500 mM glycerol, or 3 uL of benzaldehyde. | Paper_evidence | WBPaper00032335 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001602 | Paper_evidence | WBPaper00031241 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Methiothepin did not extend the life-span of animals (12% increase, n=171) as much as it extended the life-span of N2 animals (28% increase, n=273). | Paper_evidence | WBPaper00031241 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were exposed to 10uM methiothepin on day 1 of adulthood. | Paper_evidence | WBPaper00031241 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001604 | Paper_evidence | WBPaper00031241 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mianserin did not extend the life-span of animals (2% increase, n=217) as it does with N2 animals (31% increase, n=1180). | Paper_evidence | WBPaper00031241 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were exposed to 50uM mianserin starting day 1 of adulthood. | Paper_evidence | WBPaper00031241 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000643 | Paper_evidence | WBPaper00036766 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mutants grown on NGM plates exhibited superficially normal locomotion. | Paper_evidence | WBPaper00036766 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001462 | Paper_evidence | WBPaper00032335 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals exhibited wild-type chemotaxis to NaCl. | Paper_evidence | WBPaper00032335 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00003571 | Paper_evidence | WBPaper00032335 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Animals were tested for response to 25 mM NaCl. | Paper_evidence | WBPaper00032335 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001811 | Paper_evidence | WBPaper00031915 | |||||||
Curator_confirmed | WBPerson2021 | ||||||||
Remark | mod-5(n3314) mutants were further susceptible to the fat-reducing effects of exogenously administered serotonin | Paper_evidence | WBPaper00031915 | ||||||
Curator_confirmed | WBPerson2021 | ||||||||
Phenotype_assay | Treatment | Reduced fat content of 5-HT-treated animals was visualized by Nile red staining and confirmed by thin-layer chromatography (TLC) quantitation of total triglycerides extracted from vehicle- and 5-HT-treated worms and by Sudan black fat staining | Paper_evidence | WBPaper00031915 | |||||
Curator_confirmed | WBPerson2021 | ||||||||
Disease_info (2) | |||||||||
Reference (18) | |||||||||
Method | Allele |