WormBase Tree Display for Transgene: WBTransgene00000736
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| WBTransgene00000736 | Public_name | juIs167 | |
|---|---|---|---|
| Summary | [VAB-19::GFP + pRF4] | ||
| Synonym | [vab-19::gfp] | ||
| Construction | Construct | WBCnstr00000732 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | UV_TMP | ||
| Construction_summary | translational fusion with the full-length vab-19 coding sequence. Integrants of functional VAB-19::GFP arrays were obtained by TMP-UV mutagenesis. The 6.5 kb MluI-SalI genomic DNA fragment in pCZ440 was replaced with the corresponding cDNA fragment from yk481c2 to make a vab-19 minigene (pCZ437). To make the VAB-19::GFP fusion construct pCZ441, GFP coding sequence from pPD113.37 (Fire lab vector kit) was inserted in frame at the BamHI site of pCZ437. Translational fusions of the full-length vab-19 coding sequence to GFP. These VAB-19::GFP constructs completely rescued vab-19 null mutant phenotypes, suggesting that they reflect the endogenous VAB-19 expression pattern. The VAB-19::GFP transgenes displayed identical expression patterns and rescue of vab-19 phenotypes. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2823 | |
| Interactor | WBInteraction000558283 | ||
| WBInteraction000558284 | |||
| Associated_with | Strain | WBStrain00005381 | |
| Reference | WBPaper00006290 | ||
| WBPaper00035491 | |||
| WBPaper00045340 | |||
| Species | Caenorhabditis elegans |
