WormBase Tree Display for Expr_pattern: Expr2823
expand all nodes | collapse all nodes | view schema
| Expr2823 | Expression_of | Gene | WBGene00006882 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006882 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003673 | Certain | ||
| WBbt:0005733 | Certain | |||
| GO_term | GO:0005911 | |||
| GO:0005882 | ||||
| Subcellular_localization | Attachment structures contain several intermediate filament (IF) proteins, some of which are recognized by monoclonal antibody MH4. During early elongation, MH4 staining accumulated in a single patch in dorsal epidermal cells, whereas VAB-19::GFP remained diffuse. During the intermediate elongation stage, VAB-19::GFP and MH4 staining displayed more extensive colocalization. During later elongation and afterwards, VAB-19::GFP and MH4 staining fully co-localized to circumferential bands. | |||
| The transmembrane protein Myotactin maintains localization of attachment structures to muscle-adjacent parts of the epidermis. Myotactin itself initially localizes to muscle-adjacent regions of the epidermis and later becomes organized into attachment structures. VAB-19::GFP colocalized with Myotactin before the twofold stage. Both Myotactin and VAB-19::GFP localized to circumferential bands. These bands are interrupted by gaps where neuronal processes intervene between muscle and epidermis. In contrast to Myotactin or VAB-19::GFP, MH4 staining is present or enhanced at such positions. These differences suggest that VAB-19 does not colocalize with all epidermal IFs but only those associated with muscle adjacent attachment structures. | ||||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | VAB-19::GFP was first detected in embryos at the onset of elongation. At this stage, VAB-19::GFP localization was diffuse in dorsal and ventral, but not in lateral, epidermal cells. During early elongation, VAB-19::GFP started to accumulate in muscle-adjacent regions of dorsal and ventral epidermal cells. During intermediate stages of elongation, VAB-19::GFP became localized to the parts of the epidermis adjacent to body wall muscles. Finally, during the late elongation, larval and adult stages, VAB-19::GFP localized to circumferential bands in epidermis adjacent to muscles and to mechanosensory neuron processes. Within these regions, the pattern of circumferential bands is interrupted by gaps where other neuronal processes intervene between muscles and the epidermis. This pattern of localization of VAB-19::GFP resembles that of epidermal attachment structures. VAB-19::GFP was also expressed in pharyngeal marginal cells. In contrast to Myotactin, which is localized to the outer basal surface of marginal cells, VAB-19::GFP was localized throughout the apical-basal axis, although it was concentrated at the apical and basal surfaces. | |||
| Picture | WBPicture0000007572 | |||
| WBPicture0000007573 | ||||
| Remark | Transgenes containing the VAB-19 cDNA under the control of the ajm-1 promoter, which is expressed in the epidermis and other epithelial tissues but not in muscles, also fully rescued vab-19 phenotypes, consistent with VAB-19 functioning in epidermal cells. | |||
| Reference | WBPaper00006290 | |||
| Transgene | WBTransgene00000736 | |||
| Antibody_info | WBAntibody00000057 | |||
| WBAntibody00000059 |
