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WBPicture0000007573 | Description | Figure 4. VAB-19 localizes to epidermal attachment structures. (A-I) VAB-19::GFP progressively co-localizes with intermediate filaments. Confocal images of VAB-19::GFP expression (juIs167) visualized using anti-GFP immunostaining (green); intermediate filaments were visualized using the MH4 monoclonal antibody (red). At the comma stage (A-C), VAB-19::GFP partly colocalized with intermediate filaments. At the intermediate elongation stage (D-F), VAB-19::GFP and intermediate filaments both localized to muscle-adjacent regions of the dorsal and ventral epidermis. During later embryogenesis and larval stages until adulthood, staining of anti-GFP and MH4 was coincident. (G-I) L1 stage epidermis in z-axis section. (J-L) Lateral view of adult epidermis. (M-R) Colocalization of VAB-19::GFP and Myotactin (MH46 antigen). At the intermediate elongation stage (M-O), VAB-19::GFP and Myotactin are both localized to epidermal cell regions adjacent to muscle. In adults (P-R), the bands of Myotactin and VAB-19::GFP are interrupted by gaps corresponding to the positions of neuronal processes (arrows), at which positions MH4 staining is usually enhanced (K, arrow). Like Myotactin, but unlike MH4 staining, VAB-19::GFP is absent from such process gaps (compare J and P). VAB-19::GFP was also expressed in pharyngeal marginal cells (S); unlike Myotactin (T), VAB-19::GFP was localized throughout the apical basal axis of these cells and was more concentrated at the apical and basal surfaces. Scale bars: 5 um (G-I); 10 um (all others). | |||
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Name | F4.large.jpg | ||||
Depict | Expr_pattern | Expr2823 | |||
Anatomy | WBbt:0003673 | ||||
WBbt:0005733 | |||||
Cellular_component | GO:0005882 | ||||
GO:0005911 | |||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2003 | ||||
Article_URL | DOI | id | 10.1242/dev.00791 | ||
Journal_URL | Development | ||||
Publisher_URL | TheCompanyofBiologists | ||||
Reference | WBPaper00006290 |