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WormBase Tree Display for Strain: WBStrain00047484

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Name Class

WBStrain00047484EvidenceCurator_confirmedWBPerson1983
StatusLive
Genotype+/szT1 [lon-2(e678) umnIs61] I; bcat-1(ve578[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/szT1 X.
Public_nameRG3078
ContainsGeneWBGene00001149
WBGene00003056
WBGene00003514
WBGene00004496
WBGene00006789
VariationWBVar00143398
WBVar02154143
RearrangementszT1
TransgeneWBTransgene00033227
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received24 Jan 2020 00:00:00
LocationCGC
RemarkumnIs61 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] I. Homozygous Let. Deletion of 2704 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, GFP+ non-mKate2 dead larvae (ve578 homozygotes), Lon non-GFP mKate2+ males (szT1 hemizygotes), and dead eggs (szT1 homozygotes and aneuploids). Maintain by picking wild-type GFP+ mKate2+. Left flanking Sequence: ttaacacccgtatcattatcatttccatgc ; Right flanking sequence: cccaacttccttccaccccctcaaaaagcg. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
Made_by: RG KO GroupCGC_data_submission
SpeciesCaenorhabditis elegans