WormBase Tree Display for RNAi: WBRNAi00090587
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| WBRNAi00090587 | Homol | Homol_homol | R03D7:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_R03D7.1 | |||
| Experiment | Date | 08 Sep 2011 00:00:00 | |||
| Genotype | rrf-3(pk1426) | ||||
| Treatment | Synchronized L1 larvae were grown for three days on plates seeded with RNAi bacteria until control worms (grown on empty-vector control plates) reached the L4 larval stage. L4 worms were washed off the RNAi plates with M9 buffer and Beta-Ala-Lys-AMCA was added to samples to a final concentration of 0.25 mM. Worms were incubated in this solution for 3 hours at 20 degrees Celsius. Liquid OP50 culture was added to the samples and incubated with the worms for 1 hour to wash the Beta-Ala-Lys-AMCA out of the intestinal lumen. Worms were washed 4-6 times in M9 buffer and assayed for intestinal uptake of Beta-Ala-Lys-AMCA by fluorescence microscopy. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | R03D7.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00010988 | Inferred_automatically | RNAi_primary | ||
| Transcript | R03D7.1.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040287 | ||||
| Phenotype_not_observed | WBPhenotype:0002080 | Remark | RNAi does not affect the intestinal uptake of the Beta-Ala-Lys-AMCA fluorescent dipeptide, compared to controls | ||
| Affected_by | Molecule | WBMol:00005316 | |||
| Remark | (Table S1) metr-1/R03D7.1 RNAi | ||||
| Method | RNAi |
