WormBase Tree Display for Interaction: WBInteraction000544066
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| WBInteraction000544066 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00011979 | Interactor_type | Trans_regulated | |
| WBGene00018971 | Interactor_type | Trans_regulated | |||
| WBGene00015933 | Interactor_type | Trans_regulated | |||
| WBGene00007875 | Interactor_type | Trans_regulated | |||
| WBGene00009429 | Interactor_type | Trans_regulated | |||
| WBGene00010128 | Interactor_type | Trans_regulated | |||
| Other_interactor | Orthosiphon stamineus aqueous extract | Interactor_type | Trans_regulator | ||
| Interaction_summary | "we performed transcriptome analysis on a selected panel of PMK-1 and DAF- 16-regulated genes using quantitative real-time PCR. Six of the eight PMK-1-regulated genes were downregulated (~2-5-fold) following 48 hours infection by S. aureus of worms that were not treated with UE-12 (Fig. 5A). On the other hand, in infected worms treated with 200 mg/mL UE-12, five of these 6 genes - clec-67, C17H12.8, dod-24, F35E12.5 and F55G11.8 - demonstrated a 2.8-4-fold induction in expression upon supplementation with UE-12 (p<0.05), implying that UE-12 promoted the host antimicrobial response. UE-12 also significantly reduced the suppression of T24B8.5 from ~5-fold to ~2-fold (p<0.05) (Fig. 5A)." | ||||
| Detection_method | RT_PCR | ||||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Positive_regulate | Life_stage | WBls:0000041 | ||
| Paper | WBPaper00045438 | ||||
| Remark | Fig. 5a; Staphylococcus aureus infection |
