laf-1 encodes a DEAD-box RNA helicase; laf-1 is required for embryonic development and sex determination; laf-1 was identified in screens for dominant suppressors of the sterility produced by gain-of-function fem-3 mutations; when homozygous, laf-1 mutations result in embryonic and larval lethality and when heterozygous, feminization of XX and XO animals; reporter gene analyses indicate that wild-type laf-1 activity is required for proper translational repression of tra-2 mRNA via its 3'UTR; when expressed in embryos, LAF-1 localizes predominantly to the cytoplasm with additional localization seen in P granules.
Enables RNA strand annealing activity; identical protein binding activity; and molecular condensate scaffold activity. Involved in several processes, including masculinization of hermaphroditic germ-line; positive regulation of embryonic development; and positive regulation of fertilization. Located in P granule. Expressed in germ line. Human ortholog(s) of this gene implicated in colorectal cancer; hepatocellular carcinoma; and syndromic X-linked intellectual disability. Is an ortholog of human DDX3X (DEAD-box helicase 3 X-linked) and DDX3Y (DEAD-box helicase 3 Y-linked).