WormBase Tree Display for Expr_pattern: Expr1500

Expr1500 Expression_of: Gene: WBGene00003969
    Reflects_endogenous_expression_of: WBGene00003969
  Expression_data: Anatomy_term (18)
    GO_term: GO:0030425
      GO:0030424
  Subcellular_localization: FL-GFP: Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG.
    aa-(1)-NLS-GFP-LacZ: GFP-LacZ fusion protein facilitates retention in the nucleus.
  Type: Reporter_gene
  Pattern: Both: The transgene constructs showed minimal expression in non-neuronal cells. However, transgene expression in intestinal, hypodermal, or muscle cells was observed in occasional transgenic lines in which 3.0-kb genomic DNA fragments extending just past the CePPEF start codon directed production of short N-terminal regions of CePPEF fused to GFP. As these expression patterns occurred sporadically, they likely reflect transgene effects rather than an expression pattern relevant to the endogenous CePPEF gene.
    FL-GFP: FL-GFP transgene directed GFP expression to the same set of neurons as the NLS-GFP-LacZ construct, and expression in a single posterior neuron was also more clearly observed. Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG.
    aa-(1)-NLS-GFP-LacZ: Injection of the aa-(1)-NLS-GFP-LacZ construct into C. elegans revealed reporter gene expression in several anterior neurons, including AWB, AWC, AVA, AVB, AVX, BAG, and URX. The ASE neuron showed inconsistent transgene expression.
  Picture: WBPicture0000008160
    WBPicture0000008161
  Reference: WBPaper00004766
  Transgene: WBTransgene00027553