WormBase Tree Display for Expr_pattern: Expr1500
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| Expr1500 | Expression_of | Gene | WBGene00003969 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003969 | ||||
| Expression_data | Anatomy_term | WBbt:0003675 | Certain | ||
| WBbt:0003823 | Certain | ||||
| WBbt:0003824 | Certain | ||||
| WBbt:0003826 | Certain | ||||
| WBbt:0003827 | Certain | ||||
| WBbt:0003828 | Certain | ||||
| WBbt:0003829 | Certain | ||||
| WBbt:0003867 | Certain | ||||
| WBbt:0003868 | Certain | ||||
| WBbt:0003869 | Certain | ||||
| WBbt:0003870 | Certain | ||||
| WBbt:0003903 | Uncertain | ||||
| Remark | The ASE neuron showed inconsistent transgene expression. | ||||
| WBbt:0003904 | Uncertain | ||||
| Remark | The ASE neuron showed inconsistent transgene expression. | ||||
| WBbt:0004926 | Certain | ||||
| WBbt:0004928 | Certain | ||||
| WBbt:0005300 | Certain | ||||
| WBbt:0005733 | Certain | ||||
| WBbt:0005792 | Certain | ||||
| GO_term | GO:0030425 | ||||
| GO:0030424 | |||||
| Subcellular_localization | FL-GFP: Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. | ||||
| aa-(1)-NLS-GFP-LacZ: GFP-LacZ fusion protein facilitates retention in the nucleus. | |||||
| Type | Reporter_gene | ||||
| Pattern | Both: The transgene constructs showed minimal expression in non-neuronal cells. However, transgene expression in intestinal, hypodermal, or muscle cells was observed in occasional transgenic lines in which 3.0-kb genomic DNA fragments extending just past the CePPEF start codon directed production of short N-terminal regions of CePPEF fused to GFP. As these expression patterns occurred sporadically, they likely reflect transgene effects rather than an expression pattern relevant to the endogenous CePPEF gene. | ||||
| FL-GFP: FL-GFP transgene directed GFP expression to the same set of neurons as the NLS-GFP-LacZ construct, and expression in a single posterior neuron was also more clearly observed. Within the expressing cells, the FL-GFP fusion protein localized efficiently to several structures beyond the cell soma, including axons that form the ventral nerve cord; dendrites that extend to the anterior tip of the animal; and the cilia of neurons AWB, AWC, and BAG. | |||||
| aa-(1)-NLS-GFP-LacZ: Injection of the aa-(1)-NLS-GFP-LacZ construct into C. elegans revealed reporter gene expression in several anterior neurons, including AWB, AWC, AVA, AVB, AVX, BAG, and URX. The ASE neuron showed inconsistent transgene expression. | |||||
| Picture | WBPicture0000008160 | ||||
| WBPicture0000008161 | |||||
| Reference | WBPaper00004766 | ||||
| Transgene | WBTransgene00027553 |
