WormBase Tree Display for Picture: WBPicture0000008161

WBPicture0000008161 Description: Figure 3. Immunostaining with CePPEF-specific antibodies confirms the gene expression pattern and protein localization of CePPEF inferred from transgenic animals. Rabbit polyclonal antibodies were generated against the C-terminal 142 amino acids of CePPEF and affinity-purified. A, immunoblotting of 293 cells. Lane 1, untransfected cells; lane 2,CePPEF transfected cells. The bars represent (from top to bottom) the 97-, 66-, and 46-kDa markers. B, immunoblotting of C. elegans lysates prepared by sonication. Twenty micrograms of total protein were loaded in each lane. Lane 1, wild-type (N2 strain) C. elegans; lane 2, transgenic C. elegans expressing FL-GFP; lane 3, transgenic C. elegans expressing the G2A mutant of FL-GFP. The bars represent (from top to bottom) the 220-, 97-, and 66-kDa markers. C, anti-CePPEF immunofluorescent staining of wild-type C. elegans. Anterior is to the right, and the position of the sensory cilia is indicated by the arrow. Autofluorescence from gut granules is seen more caudally and is indicated by the arrowhead. The bar corresponds to 25 μm.
  Name: F3.large.jpg
  Depict: Expr_pattern: Expr1500
    Anatomy: WBbt:0005792
      WBbt:0006816
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2001
    Article_URL: DOI id 10.1074/jbc.M011712200
    Journal_URL: TheJournalofBiologicalChemistry
    Publisher_URL: TheAmericanSocietyForBiochemistryandMolecularBiology
  Reference: WBPaper00004766