WormBase Tree Display for Variation: WBVar00251300

WBVar00251300 Name: Public_name: tm2412
    Other_name: H04M03.4.1:c.748-30_910-48del
    HGVSg: CHROMOSOME_IV:g.5887932_5888301del
  Sequence_details: SMap: S_parent: Sequence: H04M03
    Flanking_sequences: ctttgagttttagtttatttgctggaccgt caacatgttatttattagttcgatgaaaca
    Mapping_target: H04M03
    Source_location: 7 CHROMOSOME_IV 5887931 5888302 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Deletion
    PCR_product: tm2412_external
      tm2412_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004715
      WBStrain00004716
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 2412
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00019154
      WBGene00173249
      WBGene00167341
    Transcript: H04M03.4.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: H04M03.4.1:c.748-30_910-48del
        Intron_number: 4-5/8
        Exon_number: 5/9
      H04M03.70
      H04M03.38
  Isolation: Mutagen: TMP/UV
  Genetics: Map: IV
  Description: Phenotype: WBPhenotype:0000010 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 worms show almost 20-fold increase in sensitivity to Proteinase K treatment Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000025 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutants have superficial blisters Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000050 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: The overall lethality is markedly accentuated, with 87.5% of tm2412 homozygotes dying during development, either as embryos or as larvae Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000054 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: Lower levels of lethality were seen in L2 larvae, and later larval stages Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000062 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: Classified as lethal OR sterile by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: FX
      WBPhenotype:0000212 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutants displayed a body constriction phenotype Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000338 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: Swollen post-anal region Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000420 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 worms displayed increased hypersensitivity to levamisole Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Affected_by: Molecule: WBMol:00004019 Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
      WBPhenotype:0000457 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutants displayed poor long-term survival on plates upon starvation Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000586 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutants are approximately five-fold more sensitive to alkaline hypochlorite exposure than WTs Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000688 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: Classified as lethal OR sterile by the National Bioresource Project of Japan. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: FX
      WBPhenotype:0001013 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1(tm2412) mutants died within hours after exposure to M. nematophilum Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001209 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutant worms exhibited a fully penetrant traction defect when moving on a bacterial lawn or on the agar surface Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Complete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001211 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 worms displayed increased permeability to dyes in comparison to WT animals Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001212 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 mutants displayed enhanced cuticle fragility Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001411 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: Plant lectins which fail to bind the surface of WT C. elegans stain glf-1 mutants with defective surface coat structures Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001418 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 worms desiccate within seconds when removed from the growth media and appear somewhat shrunken in comparison to WT Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001678 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: Loss of glf-1 activity also causes an unusual change in outer surface properties whereby E. coli cells adhere to the worm cuticle Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Penetrance: Incomplete: Paper_evidence: WBPaper00035194
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001856 Paper_evidence: WBPaper00035194
        Curator_confirmed: WBPerson2021
        Remark: glf-1 worms displayed increased hypersensitivity to ivermectin Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00035194
          Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00035194
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000103 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson712
        Remark: Comment from Dr. G. Hermann to the National Bioresource Project of Japan: wild-type gut granules. Person_evidence: WBPerson7743
            Curator_confirmed: WBPerson712
            Laboratory_evidence: GH
  Reference: WBPaper00035194
  Remark: 17190/17191-17560/17561 (370 bp deletion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele