WormBase Tree Display for Variation: WBVar00143102

WBVar00143102 Evidence: Paper_evidence: WBPaper00002911
  Name: Public_name: e307
    Other_name: T20G5.6.1:c.1280-1G>A
    HGVSg: CHROMOSOME_III:g.10184104C>T
  Sequence_details: SMap: S_parent: Sequence: T20G5
    Flanking_sequences: ggatttttctgccgcaaatttatgtttcca gaacaatgttatcatttatctggccggcact
    Mapping_target: T20G5
    Type_of_mutation: Substitution: g a Paper_evidence: WBPaper00002911
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00004145
      WBStrain00030694
    Laboratory: CB
    Status: Live
  Affects: Gene: WBGene00006783
    Transcript: T20G5.6.1 VEP_consequence: splice_acceptor_variant
        VEP_impact: HIGH
        HGVSc: T20G5.6.1:c.1280-1G>A
        Intron_number: 5/7
  Isolation: Mutagen: EMS
  Genetics: Interpolated_map_position: III 2.00018
    Mapping_data: In_2_point: 55
        62
        67
        74
        3248
      In_multi_point: 59
        61
        69
        72
        91
        1244
        1245
        1253
        1509
        1668
        2191
        2193
        2195
        2200
        2201
        2202
        3171
        3172
        3173
        3174
        3176
        3260
      In_pos_neg_data: 651
  Description: Phenotype: WBPhenotype:0000016 Paper_evidence: WBPaper00031872
        Curator_confirmed: WBPerson712
        Remark: % animals paralyzed after 60 min on 1mM aldicarb was significantly higher than % N2 animals paralyzed. Paper_evidence: WBPaper00031872
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003650 Paper_evidence: WBPaper00031872
              Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0007833 PATO:0001549 Paper_evidence: WBPaper00031872
                Curator_confirmed: WBPerson712
      WBPhenotype:0000153 Paper_evidence: WBPaper00032190
        Curator_confirmed: WBPerson712
        Remark: Animals showed strong shortening upon photoactivation of ChR2-YFP. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
          Genotype: zxIs6 Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
      WBPhenotype:0000229 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
      WBPhenotype:0000402 Paper_evidence: WBPaper00056338
        Curator_confirmed: WBPerson36360
      WBPhenotype:0000420 Paper_evidence: WBPaper00031872
        Curator_confirmed: WBPerson712
        Remark: % animals paralyzed after 60 min on 200mM levamisole was significantly higher than % N2 animals paralyzed under same conditions. Paper_evidence: WBPaper00031872
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00004019 Paper_evidence: WBPaper00031872
              Curator_confirmed: WBPerson712
      WBPhenotype:0000426 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: abnormal staining with anti-GABA antibodies (accumulates GABA) Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0000563 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: slight shrinker (contracts both dorsally and ventrally when prodded) Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0000643 Paper_evidence: WBPaper00000031
        Curator_confirmed: WBPerson48
      WBPhenotype:0000646 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: slow forward movement Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0000655 Paper_evidence: WBPaper00002911
        Curator_confirmed: WBPerson712
      WBPhenotype:0001005 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: very poor backing Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Ease_of_scoring: ES3_Easy_to_score: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0001316 Paper_evidence: WBPaper00032190
        Curator_confirmed: WBPerson712
        Remark: Light-evoked currents (photo-ePSCs) were abolished. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
          Genotype: zxIs6 Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
      WBPhenotype:0001802 Paper_evidence: WBPaper00032190
        Curator_confirmed: WBPerson712
        Remark: Animals did not elongate upon illumination (photoactivation of ChR2-YFP in GABAergic neurons), unlike wild-type animals carrying the zxIs3. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
          Photo-evoked relaxation was severely impaired. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
          Genotype: zxIs3 Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
      WBPhenotype:0002159 Paper_evidence: WBPaper00056338
        Curator_confirmed: WBPerson36360
      WBPhenotype:0004018 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: good forward movement Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
    Phenotype_not_observed: WBPhenotype:0000306 Paper_evidence: WBPaper00032190
        Curator_confirmed: WBPerson712
        Remark: Expression of ChR2-YFP in GABAergic neurons was not affected. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: zxIs3 Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
      WBPhenotype:0000567 Paper_evidence: WBPaper00032190
        Curator_confirmed: WBPerson712
        Remark: Dorsal coiling was not triggered in animals treated to photostimulation of ChR2-YFP in cholinergic neurons. Paper_evidence: WBPaper00032190
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
          Genotype: zxIs6 Paper_evidence: WBPaper00032190
              Curator_confirmed: WBPerson712
      WBPhenotype:0001645 Paper_evidence: WBPaper00040284
        Curator_confirmed: WBPerson2987
        Remark: Table 1 Paper_evidence: WBPaper00040284
            Curator_confirmed: WBPerson2987
  Reference: WBPaper00002911
    WBPaper00040284
    WBPaper00031872
    WBPaper00000031
    WBPaper00032190
    WBPaper00016473
    WBPaper00056338
  Method: Substitution_allele