Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00145224

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00145224NamePublic_nameev400
Other_nameCE04538:p.Gln99Ter
F41C6.1.1:c.295C>T
F41C6.1.2:c.295C>T
HGVSgCHROMOSOME_X:g.6890534C>T
Sequence_detailsSMapS_parentSequenceF41C6
Flanking_sequencestgtgacacttgtgatgctagaaaccatttcaatcccatccagcctctcttctaactgatc
Mapping_targetF41C6
Type_of_mutationSubstitutionctPaper_evidenceWBPaper00002378
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (69)
LaboratoryNW
StatusLive
AffectsGeneWBGene00006746
TranscriptF41C6.1.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF41C6.1.1:c.295C>T
HGVSpCE04538:p.Gln99Ter
cDNA_position361
CDS_position295
Protein_position99
Exon_number4/15
Codon_changeCaa/Taa
Amino_acid_changeQ/*
F41C6.1.2VEP_consequencestop_gained
VEP_impactHIGH
HGVScF41C6.1.2:c.295C>T
HGVSpCE04538:p.Gln99Ter
cDNA_position361
CDS_position295
Protein_position99
Exon_number4/16
Codon_changeCaa/Taa
Amino_acid_changeQ/*
Interactor (31)
IsolationMutagenEMS
GeneticsInterpolated_map_positionX-2.1004
Mapping_dataIn_multi_point4795
DescriptionPhenotype (24)
Phenotype_not_observedWBPhenotype:0000104Paper_evidenceWBPaper00033081
WBPaper00004437
Curator_confirmedWBPerson2021
WBPerson2987
RemarkPolarity is normal in all mutantsPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
"Using MIG-2::GFP, we confirmed that Q cell polarization in unc-6 mutants was also normal (data not shown)."Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBbt:0004054PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
GO_termGO:0030010PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Phenotype_assayGenotypekuIs47 [AJM-1::GFP]Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
MIG-2::GFPPaper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBPhenotype:0000220Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Remarkzmp-1 and lin-3 markers are expressed in the correct cell at the expected time in over 90% of the mutantsPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Phenotype_assayGenotypesyIs107[lin-3(delta-pes-10)::GFP], syIs49[zmp- 1::GFP]Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
WBPhenotype:0000436Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
RemarkFrom the text: "To examine whether the axonal distribution of UNC-6 by IRE-1 was required for UNC-6-mediated axon guidance, we generated worms in which UNC-6 was expressed only in the ventral neurons. We used the glr-1 promoter (Hart et al . 1995) to drive unc-6 gene expression in the ventral neurons in unc-6 null mutants. In these worms, UNC-6 was observed in the axons and cell bodies (Fig. 2D)."Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
Phenotype_assayGenotype[ghEx15(glr-1p::unc-6::Venus; tph-1p::GFP)]Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
WBPhenotype:0000469Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Remark"We found that in the null allele unc-6 (ev400) (Ishii et al., 1992), the Q cells still migrated almost as far as they do in wild type (Fig. 2)."Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBbt:0004054PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
GO_termGO:0016477PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBPhenotype:0000679Paper_evidenceWBPaper00032446
WBPaper00036484
Curator_confirmedWBPerson2021
WBPerson712
RemarkPAR-3::GFP, which localizes to apical and lateral membranes in wild-type ACs and AJM-1::GFP which marks nascent apical spot junctions, were normal in unc-6 mutantsPaper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
MADD-2::GFP was excluded from the dorsal ADL branch and was present at high levels in the cell body, a pattern that was also observed in control animals.Paper_evidenceWBPaper00036484
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005661PATO:0000460Paper_evidenceWBPaper00036484
Curator_confirmedWBPerson712
Phenotype_assayGenotypezuIs20 [par-3::GFP] , jcIs1[ajm-1::GFP]Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
WBPhenotype:0000717Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
RemarkTranscriptional reporters for FOS-1A and two of its downstream targets, ZMP-1, a matrix metalloproteinase, and hemicentin (HIM-4), a conserved extracellular matrix protein, were expressed normally in unc-6 mutantsPaper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
Phenotype_assayGenotyperhIs23[hemicentin::GFP], qyIs17[zmp-1p::mCherry], syIs77[zmp-1::YFP]Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
WBPhenotype:0001930Paper_evidenceWBPaper00032907
Curator_confirmedWBPerson712
RemarkVentral muscle arm extension in animals was indistinguishable from that of wild-type controls.Paper_evidenceWBPaper00032907
Curator_confirmedWBPerson712
Reference (40)
MethodSubstitution_allele