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WormBase Tree Display for Variation: WBVar00145224

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Name Class

WBVar00145224NamePublic_nameev400
Other_nameCE04538:p.Gln99Ter
F41C6.1.1:c.295C>T
F41C6.1.2:c.295C>T
HGVSgCHROMOSOME_X:g.6890534C>T
Sequence_detailsSMapS_parentSequenceF41C6
Flanking_sequencestgtgacacttgtgatgctagaaaccatttcaatcccatccagcctctcttctaactgatc
Mapping_targetF41C6
Type_of_mutationSubstitutionctPaper_evidenceWBPaper00002378
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (69)
LaboratoryNW
StatusLive
AffectsGeneWBGene00006746
TranscriptF41C6.1.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF41C6.1.1:c.295C>T
HGVSpCE04538:p.Gln99Ter
cDNA_position361
CDS_position295
Protein_position99
Exon_number4/15
Codon_changeCaa/Taa
Amino_acid_changeQ/*
F41C6.1.2VEP_consequencestop_gained
VEP_impactHIGH
HGVScF41C6.1.2:c.295C>T
HGVSpCE04538:p.Gln99Ter
cDNA_position361
CDS_position295
Protein_position99
Exon_number4/16
Codon_changeCaa/Taa
Amino_acid_changeQ/*
InteractorWBInteraction000500106
WBInteraction000500176
WBInteraction000500179
WBInteraction000500183
WBInteraction000500189
WBInteraction000500382
WBInteraction000500383
WBInteraction000500888
WBInteraction000500929
WBInteraction000500936
WBInteraction000501329
WBInteraction000501345
WBInteraction000501346
WBInteraction000501811
WBInteraction000502341
WBInteraction000502343
WBInteraction000503048
WBInteraction000503851
WBInteraction000503852
WBInteraction000503853
WBInteraction000518364
WBInteraction000518365
WBInteraction000518555
WBInteraction000518607
WBInteraction000519425
WBInteraction000521266
WBInteraction000521727
WBInteraction000521728
WBInteraction000524362
WBInteraction000538526
WBInteraction000538753
IsolationMutagenEMS
GeneticsInterpolated_map_positionX-2.1004
Mapping_dataIn_multi_point4795
DescriptionPhenotype (24)
Phenotype_not_observedWBPhenotype:0000104Paper_evidenceWBPaper00033081
WBPaper00004437
Curator_confirmedWBPerson2021
WBPerson2987
RemarkPolarity is normal in all mutantsPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
"Using MIG-2::GFP, we confirmed that Q cell polarization in unc-6 mutants was also normal (data not shown)."Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBbt:0004054PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
GO_termGO:0030010PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Phenotype_assayGenotypekuIs47 [AJM-1::GFP]Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
MIG-2::GFPPaper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBPhenotype:0000220Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Remarkzmp-1 and lin-3 markers are expressed in the correct cell at the expected time in over 90% of the mutantsPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
Phenotype_assayGenotypesyIs107[lin-3(delta-pes-10)::GFP], syIs49[zmp- 1::GFP]Paper_evidenceWBPaper00033081
Curator_confirmedWBPerson2021
WBPhenotype:0000436Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
RemarkFrom the text: "To examine whether the axonal distribution of UNC-6 by IRE-1 was required for UNC-6-mediated axon guidance, we generated worms in which UNC-6 was expressed only in the ventral neurons. We used the glr-1 promoter (Hart et al . 1995) to drive unc-6 gene expression in the ventral neurons in unc-6 null mutants. In these worms, UNC-6 was observed in the axons and cell bodies (Fig. 2D)."Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
Phenotype_assayGenotype[ghEx15(glr-1p::unc-6::Venus; tph-1p::GFP)]Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
WBPhenotype:0000469Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
Remark"We found that in the null allele unc-6 (ev400) (Ishii et al., 1992), the Q cells still migrated almost as far as they do in wild type (Fig. 2)."Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004056PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBbt:0004054PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
GO_termGO:0016477PATO:0000460Paper_evidenceWBPaper00004437
Curator_confirmedWBPerson2987
WBPhenotype:0000679Paper_evidenceWBPaper00032446
WBPaper00036484
Curator_confirmedWBPerson2021
WBPerson712
RemarkPAR-3::GFP, which localizes to apical and lateral membranes in wild-type ACs and AJM-1::GFP which marks nascent apical spot junctions, were normal in unc-6 mutantsPaper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
MADD-2::GFP was excluded from the dorsal ADL branch and was present at high levels in the cell body, a pattern that was also observed in control animals.Paper_evidenceWBPaper00036484
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005661PATO:0000460Paper_evidenceWBPaper00036484
Curator_confirmedWBPerson712
Phenotype_assayGenotypezuIs20 [par-3::GFP] , jcIs1[ajm-1::GFP]Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
WBPhenotype:0000717Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
RemarkTranscriptional reporters for FOS-1A and two of its downstream targets, ZMP-1, a matrix metalloproteinase, and hemicentin (HIM-4), a conserved extracellular matrix protein, were expressed normally in unc-6 mutantsPaper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
Phenotype_assayGenotyperhIs23[hemicentin::GFP], qyIs17[zmp-1p::mCherry], syIs77[zmp-1::YFP]Paper_evidenceWBPaper00032446
Curator_confirmedWBPerson2021
WBPhenotype:0001930Paper_evidenceWBPaper00032907
Curator_confirmedWBPerson712
RemarkVentral muscle arm extension in animals was indistinguishable from that of wild-type controls.Paper_evidenceWBPaper00032907
Curator_confirmedWBPerson712
Reference (40)
MethodSubstitution_allele