WormBase Tree Display for Variation: WBVar00091799

WBVar00091799 Name: Public_name: ok512
    Other_name: Y22D7AR.13.1:c.643-705_900+373del
    HGVSg: CHROMOSOME_III:g.1725264_1726599del
  Sequence_details: SMap: S_parent: Sequence: Y22D7AR
    Flanking_sequences: tgtatatacatttgaatttttgtagaaaaa aatataattttccaaaaatgaacaagtttc
    Mapping_target: Y22D7AR
    Type_of_mutation: Deletion
    PCR_product: OK512_external
      OK512_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00000209
      WBStrain00031461
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00004779
    Transcript: Y22D7AR.13.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: Y22D7AR.13.1:c.643-705_900+373del
        Intron_number: 5-6/9
        Exon_number: 6/10
  Isolation: Mutagen: UV/TMP
  Genetics: Mapping_data: In_multi_point: 4656
  Description: Phenotype (20)
    Phenotype_not_observed: WBPhenotype:0000631 Paper_evidence: WBPaper00036766
        Curator_confirmed: WBPerson712
        Remark: Sensitivity to fluoxetine-induced paralysis was not significantly changed in mutants. Paper_evidence: WBPaper00036766
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00005058 Paper_evidence: WBPaper00036766
              Curator_confirmed: WBPerson712
      WBPhenotype:0000844 Paper_evidence: WBPaper00031915
        Curator_confirmed: WBPerson2021
        Remark: Mutants were susceptible to the increased pumping rates of exogenously administered serotonin Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000041 PATO:0000460 Paper_evidence: WBPaper00031915
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: 5mM serotonin Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
          Temperature: 22C Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001012 Paper_evidence: WBPaper00032196
        Curator_confirmed: WBPerson712
        Remark: Animals were as susceptible to infection by P. aeruginosa as N2 animals. Paper_evidence: WBPaper00032196
            Curator_confirmed: WBPerson712
        Phenotype_assay: Strain: WBStrain00000209 Paper_evidence: WBPaper00032196
              Curator_confirmed: WBPerson712
      WBPhenotype:0001068 Paper_evidence: WBPaper00031241
        Curator_confirmed: WBPerson712
        Remark: Pre-exposure to mianserin blocked serotonin-induced egg laying as it does for N2 animals. Paper_evidence: WBPaper00031241
            Curator_confirmed: WBPerson712
        EQ_annotations: Life_stage: WBls:0000041 PATO:0000460 Paper_evidence: WBPaper00031241
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were pre-exposed to 50uM mianserin. Paper_evidence: WBPaper00031241
              Curator_confirmed: WBPerson712
      WBPhenotype:0001101 Paper_evidence: WBPaper00038382
        Curator_confirmed: WBPerson712
        Remark: No effects on clozapine-induced egg laying were observed. Paper_evidence: WBPaper00038382
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003634 Paper_evidence: WBPaper00038382
              Curator_confirmed: WBPerson712
      WBPhenotype:0001444 Paper_evidence: WBPaper00032335
        Curator_confirmed: WBPerson712
        Remark: Animals exhibited similar gustatory plasticity of NaCl to wild type (data not shown). Paper_evidence: WBPaper00032335
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003571 Paper_evidence: WBPaper00032335
              Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were tested for response to 25 mM NaCl after various pre-treatments: a 15-min wash in CTX buffer with or without 100 mM NaCl (liquid), or 30 min on a CTX plate with or without 100 mM NaCl, and in the presence or absence of bacteria, 500 mM glycerol, or 3 uL of benzaldehyde. Paper_evidence: WBPaper00032335
              Curator_confirmed: WBPerson712
      WBPhenotype:0001811 Paper_evidence: WBPaper00031915
        Curator_confirmed: WBPerson2021
        Remark: Mutants were susceptible to the fat-reducing effects of exogenously administered serotonin Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00031915
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: Reduced fat content of 5-HT-treated animals was visualized by Nile red staining and confirmed by thin-layer chromatography (TLC) quantitation of total triglycerides extracted from vehicle- and 5-HT-treated worms and by Sudan black fat staining Paper_evidence: WBPaper00031915
              Curator_confirmed: WBPerson2021
      WBPhenotype:0001842 Paper_evidence: WBPaper00033168
        Curator_confirmed: WBPerson2021
        Remark: Significant rebound response in ser-4 animals Paper_evidence: WBPaper00033168
            Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: trifluoperazine (40 uM) Paper_evidence: WBPaper00033168
              Curator_confirmed: WBPerson2021
  Reference (11)
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele