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WormBase Tree Display for Variation: WBVar00089401

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Name Class

WBVar00089401EvidencePaper_evidenceWBPaper00004442
NamePublic_namen301
Other_nameK10G6.1.1:c.185G>A
CE12098:p.Arg62Gln
HGVSgCHROMOSOME_II:g.3983472G>A
Sequence_detailsSMapS_parentSequenceK10G6
Flanking_sequencesgtaatgttttcaggtggcaaaactccctgcacacaacttgtcattcaacgactgcttcat
Mapping_targetK10G6
Type_of_mutationSubstitutionga
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (15)
LaboratoryMT
StatusLive
AffectsGeneWBGene00003017
TranscriptK10G6.1.1 (12)
InteractorWBInteraction000052119
WBInteraction000500693
WBInteraction000500697
WBInteraction000501874
WBInteraction000524414
IsolationMutagenEMS
GeneticsInterpolated_map_positionII-5.88214
Mapping_dataIn_2_point783
784
In_multi_point (14)
In_pos_neg_data (13)
DescriptionPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Remark21% of fertile animals (n=33) exhibited an egg laying defect.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
PenetranceIncompletePaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAll strains were grown at 20C.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
WBPhenotype:0000216Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Remarkdefect in Bγ cell fate specificationPaper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0007830PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0001708PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0000218Paper_evidenceWBPaper00026707
WBPaper00005344
WBPaper00006388
Curator_confirmedWBPerson712
WBPerson2987
Remark4.3 (n=17)Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
The lin-31(n301) mutation resulted in more than the expected number of induced vulval precursor cells (4.45 on average instead of 3.0 in wild type)Paper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
lin-31(n301) mutant animals exhibited an average of 3.81 induced vulval precursor cells (Table I), as opposed to 3.0 in wild typePaper_evidenceWBPaper00006388
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00005344
WBPaper00006388
Curator_confirmedWBPerson2987
WBPhenotype:0000239Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkVPC cells adopt 1ary/2ary/3ary fate at random. Similar phenotype in n301/Df.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Ease_of_scoringES3_Easy_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000296Paper_evidenceWBPaper00003626
Curator_confirmedWBPerson37
WBPhenotype:0000640Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
PenetranceIncomplete33% (n=212)Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
WBPhenotype:0000698Paper_evidenceWBPaper00026863
WBPaper00005344
Curator_confirmedWBPerson712
WBPerson2987
Remark33% animals (n=21) are aberrant in induction of VPCs; <3 of the VPCs, which are induced in wild type animals (P5.p to P7.p), are induced in these animals, as scored by Nomarski optics.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
5% of lin-31(n301) animals are vulvalessPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
PenetranceLowPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Probable_null_via_phenotypePaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0006748PATO:0000460Paper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAll strains were grown at 20C.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
WBPhenotype:0000700Paper_evidenceWBPaper00000762
WBPaper00026863
WBPaper00026707
WBPaper00005344
WBPaper00006388
Person_evidenceWBPerson261
Curator_confirmedWBPerson2021
WBPerson712
WBPerson2987
RemarkVulva is non-functional in some lin-31 hermaphrodites, which results in a bag of worms phenotypePaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
86% animals display one or more ectopic pseudovulvae (n=314).Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Adult hermaphrodite Muv, vulva either functional or nonfunctional protrusion; 0-4 small ventral protrusions. Similar phenotype in n301/Df.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
95% of lin-31(n301) animals have multiple vulvaPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Table IPaper_evidenceWBPaper00006388
Curator_confirmedWBPerson2987
PenetranceIncomplete74% (n=472)Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
HighPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
90% penetrantPaper_evidenceWBPaper00006388
Curator_confirmedWBPerson2987
RecessivePaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00000762
WBPaper00005344
Curator_confirmedWBPerson2021
WBPerson2987
Probable_null_via_phenotypePaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0006748PATO:0000460Paper_evidenceWBPaper00005344
WBPaper00006388
Curator_confirmedWBPerson2987
Life_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentAll strains were grown at 20C.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
WBPhenotype:0001278Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Remarkdecreased sIys145 expression in B&gamma;Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0007830PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0010467PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of B&gamma; cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/&mu;l, pBS at 20 ng/&mu;l and unc-119(+) at 40 ng/&mu;l into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0001414Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Remarkmale mating is completely abolishedPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
PenetranceHighPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Variation_effectNullPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Phenotype_assayGenotypehim-5(e1467)Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
Phenotype_not_observedWBPhenotype:0000054Paper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
WBPhenotype:0000099Paper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
RemarkThe lin-31(n301) mutation did not result in any animals with the '0 P11.p' phenotype (P11 adopts a P12 fate) or the '2 P11.p' phenotype (P12 adopts a P11 fate)Paper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
Variation_effectNullPaper_evidenceWBPaper00005344
Curator_confirmedWBPerson2987
WBPhenotype:0000170Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
WBPhenotype:0000640Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
PenetranceLow<1% (n=337)Paper_evidenceWBPaper00026707
Curator_confirmedWBPerson712
WBPhenotype:0000688Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Remark0% animals produced progeny (n=33).Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAll strains were grown at 20C.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
WBPhenotype:0000961Paper_evidenceWBPaper00038408
WBPaper00004481
Curator_confirmedWBPerson712
WBPerson2987
RemarkarIs131[lag-2p::2Xnls::yfp] is expressed specifically in P6.p in a lin-31(0) null mutant, as in wild type; arIs131[lag-2p::2nls-yfp::unc-54 3'UTR] expression remains P6.p specific.Paper_evidenceWBPaper00038408
Curator_confirmedWBPerson712
"In a lin-31 mutant background, VPCs adopt vulval or non-vulval fates randomly, although P6.p is always the closest VPC to the AC. We scored the patterning of P6.p descendants only when P6.p adopted the 1&deg; fate (scored according to detachment of the descendants from ventral cuticle and symmetry of invagination; Katz et al., 1995), and found that it displayed the wild-type zmp-1::GFP expression pattern in its progeny in all cases (n=31 animals, Table 5). Therefore, lin-31 is likely not required during 1&deg; lineage patterning."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs49 [zmp-1::GFP]Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
WBPhenotype:0001024Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkMale gross morphology wildtype. Similar phenotype in n301/Df.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Reference (13)
MethodSubstitution_allele