WormBase Tree Display for Variation: WBVar00089401

WBVar00089401 Evidence: Paper_evidence: WBPaper00004442
  Name: Public_name: n301
    Other_name: K10G6.1.1:c.185G>A
      CE12098:p.Arg62Gln
    HGVSg: CHROMOSOME_II:g.3983472G>A
  Sequence_details: SMap: S_parent: Sequence: K10G6
    Flanking_sequences: gtaatgttttcaggtggcaaaactccctgc acacaacttgtcattcaacgactgcttcat
    Mapping_target: K10G6
    Type_of_mutation: Substitution: g a
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain (15)
    Laboratory: MT
    Status: Live
  Affects: Gene: WBGene00003017
    Transcript: K10G6.1.1 (12)
    Interactor: WBInteraction000052119
      WBInteraction000500693
      WBInteraction000500697
      WBInteraction000501874
      WBInteraction000524414
  Isolation: Mutagen: EMS
  Genetics: Interpolated_map_position: II -5.88214
    Mapping_data: In_2_point: 783
        784
      In_multi_point (14)
      In_pos_neg_data (13)
  Description: Phenotype: WBPhenotype:0000006 Paper_evidence: WBPaper00026863
        Curator_confirmed: WBPerson712
        Remark: 21% of fertile animals (n=33) exhibited an egg laying defect. Paper_evidence: WBPaper00026863
            Curator_confirmed: WBPerson712
        Penetrance: Incomplete: Paper_evidence: WBPaper00026863
              Curator_confirmed: WBPerson712
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00026863
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: All strains were grown at 20C. Paper_evidence: WBPaper00026863
              Curator_confirmed: WBPerson712
      WBPhenotype:0000216 Paper_evidence: WBPaper00035553
        Curator_confirmed: WBPerson625
        Remark: defect in Bγ cell fate specification Paper_evidence: WBPaper00035553
            Curator_confirmed: WBPerson625
        EQ_annotations: Anatomy_term: WBbt:0007830 PATO:0000460 Paper_evidence: WBPaper00035553
                Curator_confirmed: WBPerson625
          GO_term: GO:0001708 PATO:0000460 Paper_evidence: WBPaper00035553
                Curator_confirmed: WBPerson625
        Phenotype_assay: Control_strain: WBStrain00044748 Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
          Treatment: ceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals. Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
          Genotype: sIys145 [ceh-13::GFP] Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
      WBPhenotype:0000218 Paper_evidence: WBPaper00026707
          WBPaper00005344
          WBPaper00006388
        Curator_confirmed: WBPerson712
          WBPerson2987
        Remark (3)
        Variation_effect: Null: Paper_evidence: WBPaper00005344
            Curator_confirmed: WBPerson2987
        EQ_annotations: Anatomy_term: WBbt:0007809 PATO:0000460 Paper_evidence: WBPaper00005344
                  WBPaper00006388
                Curator_confirmed: WBPerson2987
      WBPhenotype:0000239 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: VPC cells adopt 1ary/2ary/3ary fate at random. Similar phenotype in n301/Df. Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
        Ease_of_scoring: ES3_Easy_to_score: Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
      WBPhenotype:0000296 Paper_evidence: WBPaper00003626
        Curator_confirmed: WBPerson37
      WBPhenotype:0000640 Paper_evidence: WBPaper00026707
        Curator_confirmed: WBPerson712
        Penetrance: Incomplete: 33% (n=212) Paper_evidence: WBPaper00026707
              Curator_confirmed: WBPerson712
      WBPhenotype:0000698 Paper_evidence: WBPaper00026863
          WBPaper00005344
        Curator_confirmed: WBPerson712
          WBPerson2987
        Remark (2)
        Penetrance: Low: Paper_evidence: WBPaper00005344
              Curator_confirmed: WBPerson2987
        Variation_effect: Null: Paper_evidence: WBPaper00005344
            Curator_confirmed: WBPerson2987
          Probable_null_via_phenotype: Paper_evidence: WBPaper00026863
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006748 PATO:0000460 Paper_evidence: WBPaper00005344
                Curator_confirmed: WBPerson2987
        Phenotype_assay: Treatment: All strains were grown at 20C. Paper_evidence: WBPaper00026863
              Curator_confirmed: WBPerson712
      WBPhenotype:0000700 Paper_evidence: WBPaper00000762
          WBPaper00026863
          WBPaper00026707
          WBPaper00005344
          WBPaper00006388
        Person_evidence: WBPerson261
        Curator_confirmed: WBPerson2021
          WBPerson712
          WBPerson2987
        Remark (5)
        Penetrance: Incomplete: 74% (n=472) Paper_evidence: WBPaper00026707
              Curator_confirmed: WBPerson712
          High: Paper_evidence: WBPaper00005344
              Curator_confirmed: WBPerson2987
            90% penetrant Paper_evidence: WBPaper00006388
              Curator_confirmed: WBPerson2987
        Recessive: Paper_evidence: WBPaper00000762
          Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00000762
              WBPaper00005344
            Curator_confirmed: WBPerson2021
              WBPerson2987
          Probable_null_via_phenotype: Paper_evidence: WBPaper00026863
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006748 PATO:0000460 Paper_evidence: WBPaper00005344
                  WBPaper00006388
                Curator_confirmed: WBPerson2987
          Life_stage: WBls:0000057 PATO:0000460 Paper_evidence: WBPaper00000762
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Treatment: All strains were grown at 20C. Paper_evidence: WBPaper00026863
              Curator_confirmed: WBPerson712
      WBPhenotype:0001278 Paper_evidence: WBPaper00035553
        Curator_confirmed: WBPerson625
        Remark: decreased sIys145 expression in Bγ Paper_evidence: WBPaper00035553
            Curator_confirmed: WBPerson625
        EQ_annotations: Anatomy_term: WBbt:0007830 PATO:0000460 Paper_evidence: WBPaper00035553
                Curator_confirmed: WBPerson625
          GO_term: GO:0010467 PATO:0000460 Paper_evidence: WBPaper00035553
                Curator_confirmed: WBPerson625
        Phenotype_assay: Control_strain: WBStrain00044748 Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
          Treatment: ceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals. Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
          Genotype: sIys145 [ceh-13::GFP] Paper_evidence: WBPaper00035553
              Curator_confirmed: WBPerson625
      WBPhenotype:0001414 Paper_evidence: WBPaper00000762
        Curator_confirmed: WBPerson2021
        Remark: male mating is completely abolished Paper_evidence: WBPaper00000762
            Curator_confirmed: WBPerson2021
        Penetrance: High: Paper_evidence: WBPaper00000762
              Curator_confirmed: WBPerson2021
        Recessive: Paper_evidence: WBPaper00000762
          Curator_confirmed: WBPerson2021
        Variation_effect: Null: Paper_evidence: WBPaper00000762
            Curator_confirmed: WBPerson2021
        EQ_annotations: Life_stage: WBls:0000056 PATO:0000460 Paper_evidence: WBPaper00000762
                Curator_confirmed: WBPerson2021
        Phenotype_assay: Genotype: him-5(e1467) Paper_evidence: WBPaper00000762
              Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000054 Paper_evidence: WBPaper00005344
        Curator_confirmed: WBPerson2987
        Variation_effect: Null: Paper_evidence: WBPaper00005344
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000099 Paper_evidence: WBPaper00005344
        Curator_confirmed: WBPerson2987
        Remark: The lin-31(n301) mutation did not result in any animals with the '0 P11.p' phenotype (P11 adopts a P12 fate) or the '2 P11.p' phenotype (P12 adopts a P11 fate) Paper_evidence: WBPaper00005344
            Curator_confirmed: WBPerson2987
        Variation_effect: Null: Paper_evidence: WBPaper00005344
            Curator_confirmed: WBPerson2987
      WBPhenotype:0000170 Paper_evidence: WBPaper00026707
        Curator_confirmed: WBPerson712
      WBPhenotype:0000640 Paper_evidence: WBPaper00026707
        Curator_confirmed: WBPerson712
        Penetrance: Low: <1% (n=337) Paper_evidence: WBPaper00026707
              Curator_confirmed: WBPerson712
      WBPhenotype:0000688 Paper_evidence: WBPaper00026863
        Curator_confirmed: WBPerson712
        Remark: 0% animals produced progeny (n=33). Paper_evidence: WBPaper00026863
            Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: All strains were grown at 20C. Paper_evidence: WBPaper00026863
              Curator_confirmed: WBPerson712
      WBPhenotype:0000961 Paper_evidence: WBPaper00038408
          WBPaper00004481
        Curator_confirmed: WBPerson712
          WBPerson2987
        Remark (2)
        EQ_annotations: Anatomy_term: WBbt:0007809 PATO:0000460 Paper_evidence: WBPaper00004481
                Curator_confirmed: WBPerson2987
        Phenotype_assay: Genotype: syIs49 [zmp-1::GFP] Paper_evidence: WBPaper00004481
              Curator_confirmed: WBPerson2987
      WBPhenotype:0001024 Person_evidence: WBPerson261
        Curator_confirmed: WBPerson712
        Remark: Male gross morphology wildtype. Similar phenotype in n301/Df. Person_evidence: WBPerson261
            Curator_confirmed: WBPerson712
  Reference (13)
  Method: Substitution_allele