WormBase Tree Display for Variation: WBVar00000015

WBVar00000015 Evidence: Paper_evidence: WBPaper00031996
  Name: Public_name: ad467
    Other_name: B0365.3.2:c.1075C>T
      CE07721:p.Leu359Phe
      B0365.3.1:c.1075C>T
    HGVSg: CHROMOSOME_V:g.13128723G>A
  Sequence_details: SMap: S_parent: Sequence: B0365
    Flanking_sequences: tctaccatttgctctgataagacaggaact tcacccaaaacagaatgactgtcgctcaca
    Mapping_target: B0365
    Type_of_mutation: Substitution: c t Paper_evidence: WBPaper00031996
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00005464
      WBStrain00005507
      WBStrain00054586
    Laboratory: DA
    Status: Live
  Affects: Gene: WBGene00001137
    Transcript: B0365.3.2 (12)
      B0365.3.1 (12)
    Interactor: WBInteraction000503690
      WBInteraction000503691
      WBInteraction000518921
      WBInteraction000541752
      WBInteraction000541753
  Genetics: Interpolated_map_position: V 4.93809
    Mapping_data: In_2_point: 5213
      In_multi_point: 1837
        1839
        1840
        1841
        2403
        2404
      In_pos_neg_data: 5212
        5214
        5215
        7050
  Description: Phenotype (16)
    Phenotype_not_observed: WBPhenotype:0000655 Paper_evidence: WBPaper00031996
        Curator_confirmed: WBPerson712
        Remark: Animals responded like wild type to GABA agonist muscimol (data not shown) Paper_evidence: WBPaper00031996
            Curator_confirmed: WBPerson712
        Affected_by: Molecule: WBMol:00003906 Paper_evidence: WBPaper00031996
              Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006804 PATO:0000460 Paper_evidence: WBPaper00031996
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: Animals were treated with muscimol. Paper_evidence: WBPaper00031996
              Curator_confirmed: WBPerson712
      WBPhenotype:0000717 Paper_evidence: WBPaper00003150
        Curator_confirmed: WBPerson712
        Remark: Mutant animals did not exhibit a difference in Na, K-ATPase mRNA levels or size. Paper_evidence: WBPaper00003150
            Curator_confirmed: WBPerson712
      WBPhenotype:0001321 Paper_evidence: WBPaper00031996
        Curator_confirmed: WBPerson712
        Remark: No gross differences were observed in the pan-neuronal or GABAergic pattern or expression levels of synaptic vesicle protein SNB-1::GFP compared to wild type. Paper_evidence: WBPaper00031996
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0005829 PATO:0000460 Paper_evidence: WBPaper00031996
                Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: jsIs1 (Psnb-1::SNB-1::GFP) or juIs1 (Punc-25::SNB-1::GFP) Paper_evidence: WBPaper00031996
              Curator_confirmed: WBPerson712
      WBPhenotype:0001599 Paper_evidence: WBPaper00003150
        Curator_confirmed: WBPerson712
        Remark: Isolated Na, K -ATPase from the mutant showed similar kinetics and sensitivity to Oubain as enzyme isolated from WT worms. Paper_evidence: WBPaper00003150
            Curator_confirmed: WBPerson712
      WBPhenotype:0001685 Paper_evidence: WBPaper00031996
        Curator_confirmed: WBPerson712
        Remark: Expression of membrane inserted LEV-1 receptors was not significantly different to that observed in wild-type animals. GABA receptor localization and expression was not affected. Paper_evidence: WBPaper00031996
            Curator_confirmed: WBPerson712
        EQ_annotations: Anatomy_term: WBbt:0006804 PATO:0000460 Paper_evidence: WBPaper00031996
                Curator_confirmed: WBPerson712
        Phenotype_assay: Treatment: For assaying LEV-1 protein localization, animals were injected with fluorescently-labeled anti-HA antibodies into the body cavity (Gottschalk et al., 2005; Gottschalk and Schafer, 2006) and were observed 6 hours later. Paper_evidence: WBPaper00031996
              Curator_confirmed: WBPerson712
          Genotype: Is[Pmyo-3::UNC-29::GFP] or LEV::3xHA or oxIs22(Punc-49::UNC-49B::GFP) Paper_evidence: WBPaper00031996
              Curator_confirmed: WBPerson712
  Reference: WBPaper00014520
    WBPaper00015075
    WBPaper00027611
    WBPaper00031996
    WBPaper00001709
    WBPaper00003150
    WBPaper00050508
    WBPaper00059550
  Method: Substitution_allele