WormBase Tree Display for Transgene: WBTransgene00023641
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WBTransgene00023641 | Summary | [sur-5p::xbp-1s] | ||||||
---|---|---|---|---|---|---|---|---|
Construction | Construct | WBCnstr00038244 | ||||||
Laboratory | AGD | |||||||
Construction_summary | "DNA was injected into N2 worms at 25 ng/μl, with the exception of gly-19p::xbp-1s, which was injected at 75 ng/μl, in the presence of myo2p::tdTomato at 15 ng/μl. sur-5p::xbp-1s and rab-3p::xbp-1s extrachromosomal arrays were integrated and backcrossed to N2 seven and eight times, respectively." | |||||||
Genetic_information | Phenotype | WBPhenotype:0000136 | Paper_evidence | WBPaper00042524 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Expression of sur-5p::xbp-1s in an hsp-4p::GFP background led to robust GFP expression throughout the animal at all ages, even those at which stress-induced splicing of xbp-1 and target upregulation are no longer observed in wild-type animals (Figures 1C, 2B, and 2C). Transcript levels of UPR-ER targets were also elevated in sur-5p::xbp-1s animals (Figure 2D)." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000137 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Expression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p resulted in reduced mRNA levels of hsp-70, as determined by RT-PCR (Figure 5D) | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Expression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p resulted in reduced mRNA levels of hsp-6 and hsp-60, as determined by RT-PCR (Figure 5E) | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001171 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "However, and surprisingly, longevity of sur-5p::xbp-1s animals in the absence of ER stress was not increased; these animals exhibited a lifespan slightly shorter than that of N2 (Figure 2F)." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001236 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "Expression of sur-5p::xbp-1s in an hsp-4p::GFP background led to robust GFP expression throughout the animal at all ages, even those at which stress-induced splicing of xbp-1 and target upregulation are no longer observed in wild-type animals (Figures 1C, 2B, and 2C)." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_assay | Genotype | zcIs4 [hsp-4p::GFP] | Paper_evidence | WBPaper00042524 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0002422 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "sur-5p::xbp-1s worms had resistance to ER stress similar to that of wild-type when transferred to plates containing tunicamycin at day 1 of adulthood (Figure 2E). However, when transferred to stress conditions at day 7 of adulthood, sur-5p::xbp-1s animals lived substantially longer." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00004565 | Paper_evidence | WBPaper00042524 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Phenotype_not_observed | WBPhenotype:0000114 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Expression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p did not affect mRNA levels of hsp-16.2, as determined by RT-PCR (Figure 5D) | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000460 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "This effect was also specific, as resistance to paraquat was unaffected by sur-5p::xbp-1s expression (Figure S2F)." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Affected_by | Molecule | WBMol:00002747 | Paper_evidence | WBPaper00042524 | ||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0001006 | Paper_evidence | WBPaper00042524 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | "This difference was not caused by a reduced uptake of tunicamycin in the food, as pharyngeal pumping rates were similar in both genotypes (Figure S2E)." | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00006959 | Paper_evidence | WBPaper00042524 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Used_for | Interactor | WBInteraction000536801 | ||||||
WBInteraction000536802 | ||||||||
WBInteraction000536815 | ||||||||
WBInteraction000536816 | ||||||||
WBInteraction000536818 | ||||||||
Reference | WBPaper00042524 | |||||||
Species | Caenorhabditis elegans |