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WormBase Tree Display for Transgene: WBTransgene00023641

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Name Class

WBTransgene00023641Summary[sur-5p::xbp-1s]
ConstructionConstructWBCnstr00038244
LaboratoryAGD
Construction_summary"DNA was injected into N2 worms at 25 ng/μl, with the exception of gly-19p::xbp-1s, which was injected at 75 ng/μl, in the presence of myo2p::tdTomato at 15 ng/μl. sur-5p::xbp-1s and rab-3p::xbp-1s extrachromosomal arrays were integrated and backcrossed to N2 seven and eight times, respectively."
Genetic_informationPhenotypeWBPhenotype:0000136Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"Expression of sur-5p::xbp-1s in an hsp-4p::GFP background led to robust GFP expression throughout the animal at all ages, even those at which stress-induced splicing of xbp-1 and target upregulation are no longer observed in wild-type animals (Figures 1C, 2B, and 2C). Transcript levels of UPR-ER targets were also elevated in sur-5p::xbp-1s animals (Figure 2D)."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0000137Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
RemarkExpression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p resulted in reduced mRNA levels of hsp-70, as determined by RT-PCR (Figure 5D)Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Expression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p resulted in reduced mRNA levels of hsp-6 and hsp-60, as determined by RT-PCR (Figure 5E)Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0001171Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"However, and surprisingly, longevity of sur-5p::xbp-1s animals in the absence of ER stress was not increased; these animals exhibited a lifespan slightly shorter than that of N2 (Figure 2F)."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0001236Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"Expression of sur-5p::xbp-1s in an hsp-4p::GFP background led to robust GFP expression throughout the animal at all ages, even those at which stress-induced splicing of xbp-1 and target upregulation are no longer observed in wild-type animals (Figures 1C, 2B, and 2C)."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Phenotype_assayGenotypezcIs4 [hsp-4p::GFP]Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0002422Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"sur-5p::xbp-1s worms had resistance to ER stress similar to that of wild-type when transferred to plates containing tunicamycin at day 1 of adulthood (Figure 2E). However, when transferred to stress conditions at day 7 of adulthood, sur-5p::xbp-1s animals lived substantially longer."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000114Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
RemarkExpression of constitutively active xbp-1s from the ubiquitously expressed promoter sur-5p did not affect mRNA levels of hsp-16.2, as determined by RT-PCR (Figure 5D)Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0000460Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"This effect was also specific, as resistance to paraquat was unaffected by sur-5p::xbp-1s expression (Figure S2F)."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002747Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
WBPhenotype:0001006Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Remark"This difference was not caused by a reduced uptake of tunicamycin in the food, as pharyngeal pumping rates were similar in both genotypes (Figure S2E)."Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00006959Paper_evidenceWBPaper00042524
Curator_confirmedWBPerson2987
Used_forInteractorWBInteraction000536801
WBInteraction000536802
WBInteraction000536815
WBInteraction000536816
WBInteraction000536818
ReferenceWBPaper00042524
SpeciesCaenorhabditis elegans