WormBase Tree Display for Transgene: WBTransgene00000039
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| WBTransgene00000039 | Public_name | arIs11 | ||
|---|---|---|---|---|
| Summary | [lin-12::lacZ] | |||
| Construction | Construct | WBCnstr00000039 | ||
| Coinjection | WBCnstr00004720 | |||
| Construction_summary | This integrated array is composed of plasmids pRF4 [rol-6 (su1006)] (Mello et al., 1991) and pBGSLE (a lin-12::LacZ chimeric gene) (Wilkinson et al., 1994). pBGSLE contains all sequences required to rescue a lin-12(0)mutant (Fitzgerald et al., 1993). A modified lacZ gene (Fire et al., 1990) was inserted into the BamHI site at position 6 in frame to the lin-l2 ATG. The LacZ gene encodes a beta-galactosidase protein that contains a nuclear localization signal and includes its own stop codon but no polyadenylation signal. The mRNA encoded by the lin-12::LacZ gene is therefore predicted to be unstable due to the presence of a large 3' untranslated region containing most of the lin-12 coding sequence. For this reason beta-galactosidase activity is only detectable in the presence of a smg-1 mutation, which stabilizes mRNAs with long 3' untranslated regions (Pulak and Anderson, 1993). | |||
| Clone = pBGSLE | ||||
| Maps between dpy-17 and unc-32. | ||||
| Genetic_information | Integrated | |||
| Map | III | |||
| Map_evidence | Paper_evidence | WBPaper00002277 | ||
| Used_for | Expr_pattern | Expr1613 | ||
| Interactor | WBInteraction000502694 | |||
| WBInteraction000502695 | ||||
| Associated_with | Strain | WBStrain00008023 | ||
| Reference | WBPaper00002095 | |||
| WBPaper00002277 | ||||
| WBPaper00002412 | ||||
| WBPaper00002635 | ||||
| WBPaper00005525 | ||||
| WBPaper00006303 | ||||
| WBPaper00014840 | ||||
| WBPaper00024330 | ||||
| WBPaper00028766 | ||||
| WBPaper00035079 | ||||
| Species | Caenorhabditis elegans |
