WormBase Tree Display for Expr_pattern: Expr1613

Expr1613 Expression_of: Gene: WBGene00003001
    Reflects_endogenous_expression_of: WBGene00003001
  Expression_data: Life_stage: WBls:0000010
    Anatomy_term: WBbt:0005905 Certain
      WBbt:0006420 Certain
  Type: Reporter_gene
  Pattern: Reproducible beta-galactosidase expression was first observed in 24 cells at the 55-cell (32-AB-cell) stage in embryos carrying the lin-12::lacZ construct; variable staining was observed at the 28-, 46- and 50-cell stages. The positions of the beta-galactosidase-expressing cells suggested that all 24 expressing cells at the 55-cell stage were AB-derived. laser-ablation of AB in the 2-cell embryo eliminated all beta-galactosidase expression at a time corresponding to the wild-type 55-cell (32-AB-cell) stage. In intact transgenic embryos, beta-galactosidase expression persisted through at least one round of cell division. Again, because an engineered beta-galactosidase with a short half-life was used in the construction of the translational fusion, this observation may reflect continued expression of the lin-12 reporter. There were no reporter expression in either ABplaa or ABplpa. However, lin-12 expression was observed in the descendants of both of these cells. lin-12-expressing cells included ABplaaa and ABplpapp.
  Picture: WBPicture0000007952
  Remark: Reporter gene fusion type not specified.
  Reference: WBPaper00002635
  Transgene: WBTransgene00000039