WormBase Tree Display for Transgene: WBTransgene00000039
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WBTransgene00000039 | Public_name | arIs11 | ||
---|---|---|---|---|
Summary | [lin-12::lacZ] | |||
Construction (2) | ||||
Construction_summary | This integrated array is composed of plasmids pRF4 [rol-6 (su1006)] (Mello et al., 1991) and pBGSLE (a lin-12::LacZ chimeric gene) (Wilkinson et al., 1994). pBGSLE contains all sequences required to rescue a lin-12(0)mutant (Fitzgerald et al., 1993). A modified lacZ gene (Fire et al., 1990) was inserted into the BamHI site at position 6 in frame to the lin-l2 ATG. The LacZ gene encodes a beta-galactosidase protein that contains a nuclear localization signal and includes its own stop codon but no polyadenylation signal. The mRNA encoded by the lin-12::LacZ gene is therefore predicted to be unstable due to the presence of a large 3' untranslated region containing most of the lin-12 coding sequence. For this reason beta-galactosidase activity is only detectable in the presence of a smg-1 mutation, which stabilizes mRNAs with long 3' untranslated regions (Pulak and Anderson, 1993). | |||
Clone = pBGSLE | ||||
Maps between dpy-17 and unc-32. | ||||
Genetic_information | Integrated | |||
Map | III | |||
Map_evidence | Paper_evidence | WBPaper00002277 | ||
Used_for | Expr_pattern | Expr1613 | ||
Interactor | WBInteraction000502694 | |||
WBInteraction000502695 | ||||
Associated_with | Strain | WBStrain00008023 | ||
Reference | WBPaper00002095 | |||
WBPaper00002277 | ||||
WBPaper00002412 | ||||
WBPaper00002635 | ||||
WBPaper00005525 | ||||
WBPaper00006303 | ||||
WBPaper00014840 | ||||
WBPaper00024330 | ||||
WBPaper00028766 | ||||
WBPaper00035079 | ||||
Species | Caenorhabditis elegans |