WormBase Tree Display for Strain: WBStrain00055401
expand all nodes | collapse all nodes | view schema
| WBStrain00055401 | Genotype | npp-10(sy2071) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | ||
|---|---|---|---|---|
| Public_name | PS9999 | |||
| Contains | Gene | WBGene00000254 | ||
| WBGene00003796 | ||||
| Variation | WBVar02159155 | |||
| WBVar00143617 | ||||
| WBVar00241187 | ||||
| Rearrangement | hT2 | |||
| Transgene | WBTransgene00001901 | |||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 02 Aug 2023 00:00:00 | |||
| Location | CGC | |||
| Remark | Homozygous sterile CRISPR null mutant balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP sy2071 homozygotes (L2 or early arrested sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. CRISPR/Cas9 engineered STOP-IN null mutant of npp-10. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). Left flanking sequence: CAATCAAAACAAAGGATTGTTTGGTCAGCCAGCC. Right flanking sequence: AATAACAGTGGAACTACTGGCCTTTTCGGGGCGGC. Inserted sequence between the two-flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GTAGTTCCACTGTTATTGGC. Method Reference: Wang H, et al. G3 (Bethesda). 2018 Nov 6;8(11):3607-3616. | Inferred_automatically | From CGC strain data | |
| Made_by: Heenam Park | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
