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WormBase Tree Display for Strain: WBStrain00055041

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Name Class

WBStrain00055041Genotypetts-1(ot1234[tts-1p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) X.
Public_nameOH17942
ContainsGeneWBGene00005016
WBGene00006537
WBGene00006650
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received22 Jun 2022 00:00:00
LocationCGC
RemarkThe Null Transcriptional Reporter (NuTR) cassette was used to remove the tts-1 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in several tissues including the pharynx but is significantly upregulated in a stress-dependent manner in a variety of tissues.Inferred_automaticallyFrom CGC strain data
Made_by: Sandeep WontakalCGC_data_submission
SpeciesCaenorhabditis elegans