WormBase Tree Display for Strain: WBStrain00055041
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WBStrain00055041 | Genotype | tts-1(ot1234[tts-1p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) X. | ||
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Public_name | OH17942 | |||
Contains | Gene | WBGene00005016 | ||
WBGene00006537 | ||||
WBGene00006650 | ||||
Properties | Outcrossed | x0 | ||
Mutagen | CRISPR_Cas9 | |||
CGC_received | 22 Jun 2022 00:00:00 | |||
Location | CGC | |||
Remark | The Null Transcriptional Reporter (NuTR) cassette was used to remove the tts-1 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in several tissues including the pharynx but is significantly upregulated in a stress-dependent manner in a variety of tissues. | Inferred_automatically | From CGC strain data | |
Made_by: Sandeep Wontakal | CGC_data_submission | |||
Species | Caenorhabditis elegans |