WormBase Tree Display for Strain: WBStrain00049944
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| WBStrain00049944 | Status | Live | ||
|---|---|---|---|---|
| Genotype | cls-2(or1948)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. | |||
| Public_name | EU3020 | |||
| Contains | Gene | WBGene00000549 | ||
| WBGene00001078 | ||||
| WBGene00001609 | ||||
| Variation | WBVar00143868 | |||
| WBVar00241080 | ||||
| WBVar02157368 | ||||
| Rearrangement | qC1 | |||
| Transgene | WBTransgene00001896 | |||
| Properties | Outcrossed | x1 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 01 Sep 2020 00:00:00 | |||
| Location | CGC | |||
| Remark | Generated from papers flagged positive during the last month for data type afp_otherstrain and contains genotype and gene data. | Curator_confirmed | WBPerson1983 | |
| qIs26 [lag-2::GFP + rol-6(su1006)]. or1948 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1948 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. | Inferred_automatically | From CGC strain data | ||
| Made_by: Aleesa Schlientz | CGC_data_submission | |||
| Reference | WBPaper00060456 | |||
| Species | Caenorhabditis elegans |
