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WormBase Tree Display for Strain: WBStrain00037801

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Name Class

WBStrain00037801StatusLive
Genotypelam-3(ok2030)/hT2 I; +/hT2[bli-4(e937)] III.
Public_nameVC3567
ContainsGeneWBGene00000254
WBGene00002248
VariationWBVar00143617
WBVar00093219
RearrangementhT2
PropertiesOutcrossedx2
MutagenEMS
CGC_received07 Mar 2014 00:00:00
LocationCGC
Made_byWBPerson18852
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
T22A3.8. Homozygous lethal deletion balanced with bli-4-marked balancer. Heterozygotes are WT and segregate WT, Bli-4 hT2 homozygotes, hT2 aneuploids (arrested embryos), and ok2030 homozygotes (arrest stage/phenotype undetermined). hT2 homozygotes do not blister until the adult, and may be very difficult to tell from WT. Pick WT and check for correct segregation of progeny to maintain. External left primer: GGAGGTCGTAGATGCGAGAG. External right primer: TTCTCAACTCCGATCGCTTT. Internal left primer: TATCGGCTTCCAATCCTTTG. Internal right primer: GCTTTCGGGTAAGTGTGAGC. Internal WT amplicon: 3097 bp. Deletion size: 1499 bp. Deletion left flank: GTAAACCAGGACACGTCGGAAATCCATCTC. Deletion right flank: TGGTTCCAATATGAACCGAAAAATTTACTG.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans