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WormBase Tree Display for Strain: WBStrain00037538

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Name Class

WBStrain00037538StatusLive
Genotypepas-7(ok3447)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC2777
ContainsGeneWBGene00001072
WBGene00003928
VariationWBVar00142982
WBVar00296632
RearrangementmIn1
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenEMS
CGC_received30 Sep 2010 00:00:00
LocationCGC
Made_byWBPerson14470
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
ZK945.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok3447 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TGTGATGATCGAGGAAGCAG. External right primer: TTCGTCTCTCCCGTAAATCG. Internal left primer: AAGCAGTTGCCGCATAACTT. Internal right primer: AACGGTTCTTCTGATTTCCG. Internal WT amplicon: 1263 bp. Deletion size: 409 bp. Deletion left flank: GAATTGTGCATAAACATGTTTCTGGTTTGT. Deletion right flank: ATTCACATCCAGCTCCTCGATCTTCAGCTT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans