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WormBase Tree Display for Strain: WBStrain00037452

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Name Class

WBStrain00037452StatusLive
Genotypeerm-1(ok3269)/szT1 [lon-2(e678)] I; +/szT1 X.
Public_nameVC2617
ContainsGeneWBGene00001333
WBGene00003056
VariationWBVar00143398
WBVar00094320
RearrangementszT1
szT1[lon-2(e678)]
PropertiesOutcrossedx1
MutagenEMS
CGC_received03 Jun 2009 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C01G8.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3269 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 1068 bp. Deletion left flank: GAAAACATTTAAAAAAATGTTTATCAAAAA. Deletion right flank: CATTTTTTCGATTTTTTTTTCAGCGAAAAT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans