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WormBase Tree Display for Strain: WBStrain00037451

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Name Class

WBStrain00037451StatusLive
Genotypeerm-1(ok3268)/szT1 [lon-2(e678)] I; +/szT1 X.
Public_nameVC2616
ContainsGeneWBGene00001333
WBGene00003056
VariationWBVar00143398
WBVar00278380
RearrangementszT1
PropertiesOutcrossedx1
MutagenEMS
CGC_received25 May 2010 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C01G8.5. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3268 homozygotes. Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 528 bp. Deletion left flank: TTGTCTTGTAACAGTAGTCTGTCTCTCGAC. Deletion right flank: GTTTGTTGATACGGAGTCGTGGAGCGTAGA.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans