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WormBase Tree Display for Strain: WBStrain00036983

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Name Class

WBStrain00036983StatusLive
Genotypepbs-6&cids-1(ok2511) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Public_nameVC1946
ContainsGeneWBGene00000254
WBGene00003952
WBGene00015347
VariationWBVar00143617
WBVar00241187
WBVar00093647
RearrangementhT2
hT2[bli-4(e937) let-?(q782) qIs48]
TransgeneWBTransgene00001901
PropertiesOutcrossedx1
MutagenEMS
CGC_received14 May 2008 00:00:00
LocationCGC
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C02F5.4, C02F5.9. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2511 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AATCGAAGCGGTACTTGTGG. External right primer: CTTTCCTGCATCAAGCATCA. Internal left primer: TTTCTTCAATTGGAGGACATCT. Internal right primer: ATTCCAGGAAGATCGAGCAA. Internal WT amplicon: 2526 bp. Deletion size: 1604 bp. Deletion left flank: AATACTGGCTTACAAAATTTGAATCTTCTC. Deletion right flank: GAAGATCGAATCAAGGCAGATTTGTTAGAG. Insertion Sequence: GAATCAAGGCAGATTTGT.Inferred_automaticallyFrom CGC strain data
Made_by: Henry NgCGC_data_submission
SpeciesCaenorhabditis elegans