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WormBase Tree Display for Strain: WBStrain00036862

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Name Class

WBStrain00036862StatusLive
Genotypeapc-1(gk824)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC1771
ContainsGeneWBGene00001072
WBGene00003133
VariationWBVar00146128
WBVar00142982
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received03 Jun 2009 00:00:00
LocationCGC
Made_byWBPerson20179
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
Mutagen:UV/TMPCurator_confirmedWBPerson1983
W10C6.1. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk824 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TATGTGGAACCGAAGGAAGG. External right primer: CAGGCGTTGTTCTTTCACAA. Internal left primer: GGTCTCAGTCACCGGAGAAG. Internal right primer: ATTCAATGACCAACACGGCT. Internal WT amplicon: 2186 bp. Deletion size: 1963 bp. Deletion left flank: TGGAAGAATGCGGAGACTACACGAAAAAAT. Deletion right flank: AAAGTTATGAATTATCGTGCATTCGAGGAA.. Formerly known as mat-2.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans