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WormBase Tree Display for Strain: WBStrain00036381

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Name Class

WBStrain00036381StatusLive
Genotypelet-19&sgn-1(ok331)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC1165
ContainsGeneWBGene00001072
WBGene00002295
WBGene00004790
VariationWBVar00142982
WBVar00091629
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received08 Jun 2006 00:00:00
LocationCGC
Made_byWBPerson20180
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F07H5.2, K08F8.6. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok331 homozygotes (grotty sterile Dpy with vulval blip). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TGAAAATTGGGAGTTCGGAG. External right primer: ACCACTTGTTCCTTGCCAAC. Internal left primer: GGACTGGAAACTCCAAGCAG. Internal right primer: GACTGATGAGCCGGTATGGT. Internal WT amplicon: 2637 bp. Deletion size: 1456 bp.Inferred_automaticallyFrom CGC strain data
Mutagen:UV/TMPCurator_confirmedWBPerson1983
SpeciesCaenorhabditis elegans