WormBase Tree Display for Strain: WBStrain00036124
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| WBStrain00036124 | Status | Live | ||
|---|---|---|---|---|
| Genotype | eif-3.H(ok1353)/szT1 [lon-2(e678)] I; +/szT1 X. | |||
| Public_name | VC856 | |||
| Contains | Gene | WBGene00001231 | ||
| WBGene00003056 | ||||
| Variation | WBVar00143398 | |||
| WBVar00092578 | ||||
| Rearrangement | szT1 | |||
| szT1[lon-2(e678)] | ||||
| Properties | Outcrossed | x1 | ||
| Mutagen | UV+TMP | |||
| CGC_received | 25 Mar 2009 00:00:00 | |||
| Location | CGC | |||
| Made_by | WBPerson3820 | |||
| Remark | This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. | Paper_evidence | WBPaper00041807 | |
| C41D11.2. Homozygous sterile deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, arrested szT1 aneuploids, Lon-2 males, and ok1353 homozygotes (sterile adult). Pick WT and check for correct segregation of progeny to maintain. Gravid WT progeny that do not segregate Lon-2 males are rare recombinants. External left primer: ATGATGGTGGTGGGATTGTT. External right primer: GGGGAAGGTGGAAAAGGATA. Internal left primer: TGGAACCAATGGTGTCTGAA. Internal right primer: GGGAGGAAACAAAAACACGA. Internal WT amplicon: 2150 bp. Deletion size: 1337 bp. Deletion left flank: GTGAACTTCATGCAGGAATTAGTGAGGTAT. Deletion right flank: GCTGTTGCTGAGGAGAAAGTCGCCGGAACA. Insertion Sequence: GT. | Inferred_automatically | From CGC strain data | ||
| Made_by: Anna Rankin | CGC_data_submission | |||
| Mutagen:UV/TMP | Curator_confirmed | WBPerson1983 | ||
| Species | Caenorhabditis elegans |
