WormBase Tree Display for RNAi: WBRNAi00106944
expand all nodes | collapse all nodes | view schema
| WBRNAi00106944 | Homol | Homol_homol | Y53C12B:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | mv_Y53C12B.2 | |||
| Experiment | Genotype | Pacdh-1::GFP | |||
| Treatment | "To identify additional genes that can affect the dietary sensor, we explored the use of RNAi by feeding, which is carried out in E. coli HT115 bacteria (Timmons et_al, 2001). When fed E. coli HT115, the Pacdh-1::GFP animals express intermediate levels of GFP, enabling us to identify both dietary sensor repressors and activators. In addition, we took advantage of the fact that Comamonas DA1877 can be diluted and still repress the dietary sensor (MacNeil et_al, 2013). Adding a small amount of Comamonas DA1877 to the E. coli HT115 RNAi feeding lawn repressed the dietary sensor while maintaining RNAi knockdown efficiency (Figure 2A). This enabled us to perform a genome-scale RNAi screen in the presence of Comamonas DA1877 (Figure 2B)." | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | Y53C12B.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00013144 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y53C12B.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00042204 | ||||
| Phenotype | WBPhenotype:0000031 | Remark | RNAi results in undetectable expression of the Pacdh-1::GFP transgene | ||
| WBPhenotype:0001375 | Remark | RNAi results in undetectable expression of the Pacdh-1::GFP transgene | |||
| Remark | (Table S2) Y53C12B.2 RNAi | ||||
| Method | RNAi |
