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WormBase Tree Display for RNAi: WBRNAi00096402

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Name Class

WBRNAi00096402HomolHomol_homolC16A3:RNAi
Sequence_infoPCR_productsjj_C16A3.1
ExperimentLaboratoryGR
Date23 Feb 2012 00:00:00
StrainWBStrain00034065
TreatmentFor reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC16A3.1cInferred_automaticallyRNAi_primary
C16A3.1bInferred_automaticallyRNAi_primary
C16A3.1aInferred_automaticallyRNAi_primary
GeneWBGene00015806Inferred_automaticallyRNAi_primary
TranscriptC16A3.1b.1Inferred_automaticallyRNAi_primary
C16A3.1a.1Inferred_automaticallyRNAi_primary
C16A3.1c.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00040984
Phenotype_not_observedWBPhenotype:0001236RemarkRNAi does not result in activation of the hsp-4::GFP reporter gene
Remark(Table S2B) C16A3.1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi