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WormBase Tree Display for Strain: WBStrain00034065

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Name Class

WBStrain00034065StatusLive
GenotypezcIs4 V.
Public_nameSJ4005
ContainsTransgeneWBTransgene00005180
PropertiesOutcrossedx5
MutagenUV+TMP
CGC_received16 Sep 2002 00:00:00
PhenotypeWBPhenotype:0000083Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
RemarkAt the 10-hour time point, when the majority of control animals had progressed to the early L4, tunicamycin-treated animals at both 20C and 25C were arrested at L3.Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Phenotype_assayTemperature20 deg C; 25 deg CPaper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
GenotypezcIs4[Phsp-4::GFP; lin-15(n765)]Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
WBPhenotype:0000757Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
RemarkMany animals had molting problems, like not exiting the old cuticle after shedding, or appearing to start ecdysis but bursting open during the process. At 24 hours, when the majority of control animals were young adults, tunicamycin-treated animals were still developmentally delayed. A considerable fraction were still at L3, and many animals had cuticle shedding abnormalities. Overall, there appeared to be more dead animals on the tunicamycin plates at 25C compared to those grown at 20C, but this was not quantified directly.Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Phenotype_assayTemperature20 deg C; 25 deg CPaper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
GenotypezcIs4[Phsp-4::GFP; lin-15(n765)]Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
WBPhenotype:0001834Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
RemarkThey exhibited a sharp increase in xbp-1 splicing after 2 hours of exposure (Figure 1A and B, compare 2 hours + tunicamycin to the 0-hour control). The maximal splicing seen at 2 hours decreased steadily for the duration of the time course at both temperatures. The attenuation of xbp-1 splicing occurred faster in animals grown at 25C (Figure 1B), where the reduction became statistically significant at 10 hours of exposure to tunicamycin (compare 2 and 10 hours + tunicamycin). In contrast, the reduction did not become significant in animals grown at 20C until 24 hours of exposure (Figure 1A, compare 2 and 24 hours + tunicamycin). Control animals grown without tunicamycin did not exhibit spliced xbp-1 above background levels throughout the time course (Figure 1A and B, - tunicamycin).Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004565Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25 deg CPaper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
Phenotype_assayGenotypezcIs4[Phsp-4::GFP; lin-15(n765)]Paper_evidenceWBPaper00053292
Curator_confirmedWBPerson712
LocationCGC
SJ
Made_byWBPerson4409
Remark (15)
Reference (19)
SpeciesCaenorhabditis elegans