WormBase Tree Display for Strain: WBStrain00034065
expand all nodes | collapse all nodes | view schema
WBStrain00034065 | Status | Live | ||||||
---|---|---|---|---|---|---|---|---|
Genotype | zcIs4 V. | |||||||
Public_name | SJ4005 | |||||||
Contains | Transgene | WBTransgene00005180 | ||||||
Properties | Outcrossed | x5 | ||||||
Mutagen | UV+TMP | |||||||
CGC_received | 16 Sep 2002 00:00:00 | |||||||
Phenotype | WBPhenotype:0000083 | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | At the 10-hour time point, when the majority of control animals had progressed to the early L4, tunicamycin-treated animals at both 20C and 25C were arrested at L3. | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004565 | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Temperature | 20 deg C; 25 deg C | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | zcIs4[Phsp-4::GFP; lin-15(n765)] | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000757 | Paper_evidence | WBPaper00053292 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Many animals had molting problems, like not exiting the old cuticle after shedding, or appearing to start ecdysis but bursting open during the process. At 24 hours, when the majority of control animals were young adults, tunicamycin-treated animals were still developmentally delayed. A considerable fraction were still at L3, and many animals had cuticle shedding abnormalities. Overall, there appeared to be more dead animals on the tunicamycin plates at 25C compared to those grown at 20C, but this was not quantified directly. | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004565 | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Temperature | 20 deg C; 25 deg C | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Genotype | zcIs4[Phsp-4::GFP; lin-15(n765)] | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001834 | Paper_evidence | WBPaper00053292 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | They exhibited a sharp increase in xbp-1 splicing after 2 hours of exposure (Figure 1A and B, compare 2 hours + tunicamycin to the 0-hour control). The maximal splicing seen at 2 hours decreased steadily for the duration of the time course at both temperatures. The attenuation of xbp-1 splicing occurred faster in animals grown at 25C (Figure 1B), where the reduction became statistically significant at 10 hours of exposure to tunicamycin (compare 2 and 10 hours + tunicamycin). In contrast, the reduction did not become significant in animals grown at 20C until 24 hours of exposure (Figure 1A, compare 2 and 24 hours + tunicamycin). Control animals grown without tunicamycin did not exhibit spliced xbp-1 above background levels throughout the time course (Figure 1A and B, - tunicamycin). | Paper_evidence | WBPaper00053292 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004565 | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Temperature_sensitive | Heat_sensitive | 25 deg C | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Genotype | zcIs4[Phsp-4::GFP; lin-15(n765)] | Paper_evidence | WBPaper00053292 | ||||
Curator_confirmed | WBPerson712 | |||||||
Location | CGC | |||||||
SJ | ||||||||
Made_by | WBPerson4409 | |||||||
Remark (15) | ||||||||
Reference (19) | ||||||||
Species | Caenorhabditis elegans |