WormBase Tree Display for RNAi: WBRNAi00088408
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| WBRNAi00088408 | Homol | Homol_homol | F22B7:RNAi | |||
|---|---|---|---|---|---|---|
| C38C10:RNAi | ||||||
| Sequence_info | DNA_text | ATGGACGTCTCTTATTACGATGGTCCCAAGGATGAAGTCGCCGAAGCAATGCTGAAAAGCGCGGTGACGGCCATGAGATTGGGACAATACGAGGATGGAAAAGGACGCTTAGAGGAGATAATGGAGTTCGGAACCTCAAATTTTCAACTACTTGGTACAATCTACATGTATTACGGAAGAGTGTGCAGGCATTTGAACCATGATGCCAAGGCCTTGGAGTTTTTCGAACATGAGTTGAACATGTTCAAATTGATCTTCAACTACCCAGAAGCATGTGATTCCACACGTCGCATCGTCGAGCAGGCACTCAAAATGGGAAAGTTCCCCAAGGCTCGACGGTTTGCTGAGGATCTCATTGATTACACCAGCAATAAGAAGAACGGAGAGAAGTATATCGGTCAAGCTCGAATTTTGTTCGCTTCCGTGTGCCTCGAAGGATGTGAAAGAGACGTCGAGAGTAATCAAGATGAGAAGAAGAAGCTTTTGTCAATATGTGCTGAACAGATTGCAGCCGTGAAATTGTTCAACGAGAATAATACGGAAGGAGCTGTGTCTGAGACCAAAATCATGTTACTTGAGGCGAAATGCTTGTCACTAGACGAAAAATACGAGGAATCGCGTCGCAAGTATCAAGAATGCATCGATTTTGCCATCAAAACAGACCAGTTTGAAGCAGTTCACATCGCCTATTACGACAAGGCTCTATATGCTGAGACAGATCTTCTTTTCTTTATTATCAGAGATCTCAGAAGTGCTCTCTTCTACGCCACGAAATTCGGAAAAGAGCGAGATGTAGTCAAATATAAGTCGAAGCTATCCGAAGAGATGCTGAGAAATGGCGAATTCCACGAAGCATATCTCTACGGATTGGAAGCGCTTGTATCGATTCGGAAGCTTGGATTGAACGAATACATTGGAGATGTGTTGCTTACAATCGCAAAGTGCCTCATTGCACTTGGAAAAAGACGCCAAGCTGCTTATTTTATCATCTTGGGGAGTGTTCTGACCATCAACCAAAACAGTTTCAAACTGTTCTACGAGCAGATCGACGTGGCGATGAATCAAGAGAGAAGCGAAACGGCAACTGATCAAGATGTATGCCTCGCAATTGATTCGTCTCCTGATCCGACATCCTCGAATGACATGATTAATAAGTTCGTCGTCGAACTGGAGCACGCAACAAATGTGGAAACCTGGGAAATGATTGTCAACGGAATCATTGACGACCAGAAGAAACCAGTGGCGATCGAAAAGAAAGAGAACGAGGAACCCGTAGACATGATGGATCTCATTTTCAGTATGAGCTCACGTATGGATGATCAAAGAACTGAACTGCCTGCTGCCAGATTCATTCCGCCTCGTCCAGTGTCATCGGCATCGAAAAAGACTACAAAGAGTCACAGAATCCTCCCTGGACTCCGTGCCAATTGGACAAAAGTGCAGTCGATGAAGTTCGATGGTCACACAATGAATAGGATCCTGAAGAGGTCGAAGAAAAGCAAATCGTCATTGGATTCTACAAATTCGATGCAGGGCGATGATACTCGAAGCGATGATGTGACAATGACGTCCAAATAG | ||||
| Experiment | Laboratory | GZ | ||||
| Date | 20 Jun 2003 00:00:00 | |||||
| Strain | WBStrain00000001 | |||||
| Delivered_by | Injection | |||||
| Inhibits | Predicted_gene | C38C10.4 | Inferred_automatically | RNAi_primary | ||
| F22B7.13 | Inferred_automatically | RNAi_primary | ||||
| Gene | WBGene00001689 | Inferred_automatically | RNAi_primary | |||
| WBGene00001688 | Inferred_automatically | RNAi_primary | ||||
| Transcript | C38C10.4.1 | Inferred_automatically | RNAi_primary | |||
| F22B7.13.1 | Inferred_automatically | RNAi_primary | ||||
| Species | Caenorhabditis elegans | |||||
| Reference | WBPaper00005918 | |||||
| Phenotype | WBPhenotype:0001959 | Remark | In wild-type one-cell stage embryos, the spindle elongates asymmetrically toward the posterior during anaphase, accompanied by vigorous transverse movements of the posterior spindle pole, which flattens at telophase. As a result, the first division is unequal, generating a larger anterior blastomere and a smaller posterior one. In gpr-1/2(RNAi) one-cell stage embryos, the spindle did not elongate asymmetrically during anaphase in 25 of 30 cases. As a result, the first division was equal, generating two blastomeres of identical size. In the other five gpr-1/2(RNAi) embryos, the posterior spindle pole underwent weak posterior displacement, which, although clearly distinguishable from the wild type, resulted in unequal first division. In all gpr-1/2(RNAi) embryos, transverse movements of the posterior spindle pole and its flattening at telophase did not take place. Other defects were apparent when gpr-1 and gpr-2 were inactivated by RNAi, including aberrant centrosome and spindle positioning in two-cell stage embryos as well as occasional chromosome segregation defects. | |||
| EQ_annotations | Life_stage | WBls:0000006 | PATO:0000460 | |||
| Remark | paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gpr-1 used for curation. | |||||
| Method | RNAi |
