WormBase Tree Display for Picture: WBPicture0000011729

WBPicture0000011729 Description: Figure 1 Tissue distribution of small heat-shock proteinsIn all panels anterior is on the left and dorsal towards the top. Small heat-shock proteins were labelled with rabbit polyclonal antibodies as previously described [2], and the images are shown in green. (A) HSP12s (HSP12.6 antibody). A1, overview of HSP12 expression in L4 hermaphrodite. Spermathecae are strongly labelled and vulva is lightly labelled (arrows), with nuclei (red, DAPI) showing the positions of the tissues. A2-A5, staining in spermatheca (A2) is confirmed by co-staining of sperm nuclei (A3; red, DAPI). The images in A2 and A3 are merged in A4. A5, HSP12s localized in cytoplasm of sperm cells (green), with nuclei shown in red (DAPI). A6-A8, adult male showing HSP12s in sperm (S). The positions of sperm cells are indicated by nuclear counterstaining with DAPI (A7), and the distal loop of the testis (T) is indicated. Images A6 and A7 are merged in A8. A9-A12, anti-HSP12 labels vulva in adult hermaphrodites. A9 and A10, staining pattern of HSP12s in vulval region. A11, vulval muscles are revealed by counterstaining with a monoclonal antibody to myosin heavy chain, DM5.6 [16]. Images A10 and A11 are merged in A12. A13, HSP12s are ubiquitously expressed in L1 (green) with nuclei shown in red (DAPI). A14 and A15, controls: HSP12.6 antibody was pre-incubated with an excess of recombinant HSP12.3. The nematode was counterstained with DAPI to show nuclei, and with DM5.6 to label myosin. (B) HSP16 localization with polyclonal anti-HSP16-2 antibody [15]. B1, in late L1, HSP16s are ubiquitously expressed, although particularly prominent in nerve ring (arrow). B2, adult hermaphrodite: in addition to general tissue staining, anti-HSP16 strongly labels spermathecae (SP), and, more lightly, the vulval region (V). B3, HSP16s (green) are localized in sperm cytoplasm, with nuclei shown in red (DAPI). B4 and B5, HSP16s (green) are concentrated in discrete cytoplasmic regions in intestinal cells and along the border of the lumen (L); large intestinal nuclei (arrows, B5) are shown in red (DAPI). B6, HSP16s are expressed throughout the body in males, slightly concentrated over sperm (S). Sperm nuclei were stained with DAPI (B7), and the images are merged in B8. B9, unstressed L2-L3 animal, showing lack of HSP16 staining (green). The animal was counterstained with DAPI, and nuclei are shown in blue. B10, as a control for permeabilization, the animal in B9 (DAPI nuclei in blue) was also counterstained with monoclonal antibody DM5.6 [15] to show body-wall muscle (red). (C) HSP43. C1, overview of HSP43 staining pattern in male. HSP43 staining is conspicuous in the tail (T) and at hypodermal/muscle cell contacts (H), and less prominent over the location of spermatids in the testis (S). C2, anti-HSP43 labels tail rays in males (R and arrows, inset). Nuclei (red, DAPI) show the position of the tissues. C3, tail region of male, with anti-HSP43 labelling of vas deferens or, possibly, copulatory spicules (arrows). C4, HSP43 labels the hook and other structures in the male tail (arrow). C5, hermaphrodite, showing anti-HSP43 labelling of spermathecae and vulva.
  Name: FigB.jpg
  Depict: Expr_pattern: Expr1116
      Expr1117
      Expr1118
    Anatomy (11)
    Cellular_component: GO:0005634
      GO:0005737
  Acknowledgment: Template: Reproduced with permission, from <Article_URL>, <Publication_year>, <Journall_URL>. Copyright <Publisher_name>.
    Publication_year: 2000
    Article_URL: DOI id 10.1042/0264-6021:3510013
    Journal_URL: BiochemicalJournal
    Publisher_URL: PortlandPressLTD
  Reference: WBPaper00004387