WormBase Tree Display for Expr_pattern: Expr1116
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| Expr1116 | Expression_of | Gene | WBGene00002013 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002013 | ||||
| Expression_data | Life_stage | WBls:0000024 | |||
| WBls:0000038 | |||||
| WBls:0000041 | |||||
| Anatomy_term | WBbt:0005821 | Partial | |||
| Remark | Expressed in a subset of vulval muscle cells. | ||||
| WBbt:0006798 | Certain | ||||
| WBbt:0006799 | Uncertain | ||||
| WBbt:0006800 | Certain | ||||
| GO_term | GO:0005737 | ||||
| Subcellular_localization | At higher magnification, the distribution of HSP12 cytoplasmic staining in sperm cells is seen to be spherically symmetrical. | ||||
| Type | Antibody | Rabbit anti-HSP12.6. | |||
| Pattern | immunohistochemistry shows that L1 larvae have high HSP12 levels throughout the body. Although overall levels of HSP12s are much lower in L4 larvae and in adult hermaphrodites, these smHSPs are locally abundant in the spermatheca and in specific vulval cells under unstressed conditions. In the spermatheca of hermaphrodites, HSP12s are localized in sperm. At higher magnification, the distribution of HSP12 cytoplasmic staining in sperm cells is seen to be spherically symmetrical. In males, spermatids are stored in the seminal vesicle, and most sperm cells mature following ejaculation; the extensive labelling of HSP12 in male germ cells therefore indicates localization of this smHSP to spermatids and perhaps spermatocytes. Cells in the mitotic region of the male gonad, however, are not labelled. In hermaphrodites, HSP12s are also expressed in a subset of vulval muscle cells (A9A12). The specificity of the antibody for HSP12s is demonstrated by the loss of signal when the antibody is pre-incubated with excess recombinant HSP12.3. | ||||
| Picture | WBPicture0000011728 | ||||
| WBPicture0000011729 | |||||
| WBPicture0000011730 | |||||
| Reference | WBPaper00004387 | ||||
| Antibody_info | WBAntibody00000323 |
