WormBase Tree Display for Expr_pattern: Expr1117
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| Expr1117 | Expression_of | Gene | WBGene00002016 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002016 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0005319 | Certain | ||
| WBbt:0005772 | Certain | |||
| WBbt:0006748 | Certain | |||
| WBbt:0006798 | Certain | |||
| WBbt:0006800 | Certain | |||
| GO_term | GO:0005737 | |||
| Subcellular_localization | Counterstaining with DAPI revealed HSP16 localized in discrete regions within the cytoplasm of intestinal cells, characterized by their large nuclei. Concentrations of HSP16-2 occur along the luminal border of these cells. | |||
| Type | Antibody | polyclonal anti-HSP16-2 antibody before and after heat-shock. | ||
| Reporter_gene | ||||
| Pattern | HSP16-2 is ubiquitously expressed throughout most somatic tissues of larvae after heat-shock, and the intensity gradually decreased with age. HSP16 labelling is prominent in spermathecae and in the vulval region in L4 and adult hermaphrodites. The spermathecal labelling seems to consist of cytoplasmic localization of HSP16-2 in sperm, and perhaps also in the spermathecal cage cells. The prominent localization of HSP16 to intestinal cells was previously noted in transgenic animals expressing a lacZHSP16 gene fusion. Vulval expression of HSP16 was also seen in transgenic reporter experiments. In males, HSP16-2 was distributed more generally, though it was slightly concentrated in the lower testis in the region corresponding to spermatids, and in tail structures. The latter expression pattern was also seen with transgenic reporter constructs. | |||
| Picture | WBPicture0000011728 | |||
| WBPicture0000011729 | ||||
| WBPicture0000011730 | ||||
| Remark | Reporter gene fusion type not specified. | |||
| Similar patterns of HSP16 distribution were observed with two other polyclonal antibodies, one with greater specificity for HSP16-41 and another which cross-reacts with all four stress-inducible HSP16s. Thus the staining pattern described here reflects the distribution of the HSP16 family in general. Non-stressed animals showed little or no detectable signal. | ||||
| Reference | WBPaper00004387 | |||
| Transgene | WBTransgene00027351 |
