WormBase Tree Display for Picture: WBPicture0000009923

WBPicture0000009923 Description: (D-G) Immunolocalization of SYP-2 throughout meiotic prophase. Images in (D), (E), and (G) show nuclei from whole-mount preparations of wild-type germlines; images in (F) show two halves of a single nucleus prepared using a squash procedure. DAPI-stained chromosomes are in blue; A-SYP-2 is in red.(D) Field of nuclei that spans entry into meiotic prophase; progression is from left to right. The first nuclei in which SYP-2 is detected contain a single bright SYP-2 focus; these are followed by nuclei with several chromosomal foci of SYP-2, then by nuclei with extended chromosome-associated patches of SYP-2 by mid-transition zone.(E) Pachytene nuclei, exhibiting continuous SYP-2 localization at the interface between aligned homologous chromosomes; images are projections halfway through data stacks encompassing whole nuclei.(F) Nucleus exhibiting extensive synapsis over most chromosome segments (indicated by tight association of parallel DAPI tracks flanking A-SYP-2 staining) plus a region in which corresponding segments of homologs are not closely juxtaposed and lack A-SYP-2 staining (arrow).(G) Three nuclei at the diakinesis stage, in which chromosomes are highly compact and individual bivalents are clearly resolved. SYP-2 progressively dissociates from chromosomes during diakinesis and is no longer detected by late diakinesis (progression is from left to right). Scale bars equal 2 um.
  Name: S2_A.jpg
  Crop: Cropped_from: WBPicture0000009922
  Depict: Expr_pattern: Expr2680
    Cellular_component: GO:0005634
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2003
    Article_URL: DOI id 10.1016/S1534-5807(03)00232-6
    Journal_URL: DevelopmentalCell
    Publisher_URL: Elsevier
  Reference: WBPaper00006086